WormBase Tree Display for Transgene: WBTransgene00007377
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| WBTransgene00007377 | Public_name | tvEx35 | |||||||
|---|---|---|---|---|---|---|---|---|---|
| Summary | [Phsp-16.2::HA::RNF-121; myo-2p::GFP] | ||||||||
| Construction | Construct | WBCnstr00007193 | |||||||
| Coinjection_other | myo-2p::GFP | ||||||||
| Construction_summary | This heat-shock inducible construct was generated by cloning the full-length rnf-121 cDNA with an N-terminal HA tag into the pPD49.78 vector which contains the hsp-16.2 promoter site (A. Fire) (Darom et al., 2010). | ||||||||
| Genetic_information | Extrachromosomal | ||||||||
| Phenotype | WBPhenotype:0000105 | Paper_evidence | WBPaper00036076 | ||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Remark | "Meiotic maturation rates were slower ( 0.85 +/- 0.23 maturations / gonad arm / h ; n = 27 ) relative to the maturation rates in wild-type hermaphrodite that were treated with the same heat-shock conditions ( 2.65 +/- 0.50 maturations / gonad arm / h ; n = 16 ) ." | Paper_evidence | WBPaper00036076 | ||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Caused_by_gene | WBGene00007626 | Paper_evidence | WBPaper00036076 | ||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| WBPhenotype:0000186 | Paper_evidence | WBPaper00036076 | |||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Remark | "DIC and 4,6-diamidino-2- phenylindole ( DAPI ) analyses revealed delayed develop- ment of the oocytes in the proximal gonads ( Figure 4 , C-E , compared with Figure 4 , A and B ) . In wild-type gonad , progression of nuclei from pachytene into diplotene occurs in the loop region , whereas in the proximal gonad arm oocytes progress to diakinesis ( Figure 4B , short arrow ) . In contrast , pachytene nuclei were found in the hsp-16p : : RNF-121 proximal gonads after the loop region , indicating a delay in progression into diplotene ( 72.6 +/- 4.5% ; n = 256 ) ( Figure 4E , arrowhead ) ." | Paper_evidence | WBPaper00036076 | ||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Caused_by_gene | WBGene00007626 | Paper_evidence | WBPaper00036076 | ||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| WBPhenotype:0000388 | Paper_evidence | WBPaper00036076 | |||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Remark | " the sperm in the hsp-16 : : RNF-121 adults seem abnormal and swollen ( Fig- ure 4F , arrow )" | Paper_evidence | WBPaper00036076 | ||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Caused_by_gene | WBGene00007626 | Paper_evidence | WBPaper00036076 | ||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| WBPhenotype:0000690 | Paper_evidence | WBPaper00036076 | |||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Remark | "In addition to abnormalities in oocyte development and maturation after induction of RNF-121 , we observed gonad migration defects that involved extra turns and abnormal migration paths , as a 45 migration toward the dorsal side followed by a reversed migration to the ventral side and repeated migration toward the dorsal side ( Figure 4G ) or a reversed migration on the ventral side till the midbody ( Figure 4H ) ." | Paper_evidence | WBPaper00036076 | ||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Caused_by_gene | WBGene00007626 | Paper_evidence | WBPaper00036076 | ||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| WBPhenotype:0001213 | Paper_evidence | WBPaper00036076 | |||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Remark | "Moderate decrease in worm motility was detected following RNF-121 expression in adults ( Supplemental Figure S3 ) ." | Paper_evidence | WBPaper00036076 | ||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Caused_by_gene | WBGene00007626 | Paper_evidence | WBPaper00036076 | ||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| WBPhenotype:0001259 | Paper_evidence | WBPaper00036076 | |||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Remark | "We observed defects in normal development only when RNF-121 was induced at a specific developmental stage , the mid-L2 stage , resulting in decreased hermaphrodite fertility . The average number of progeny of hsp-16p : : RNF-121 was 55 +/- 18 ( n = 65 ) compared with 254 +/- 28 ( n = 60 ) in wild-type worms treated with the same heat-shock conditions at the mid-L2 stage ( Supplemental Figure S4 ) . " | Paper_evidence | WBPaper00036076 | ||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Caused_by_gene | WBGene00007626 | Paper_evidence | WBPaper00036076 | ||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| WBPhenotype:0001260 | Paper_evidence | WBPaper00036076 | |||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Remark | " In addition , the developing oocytes and especially the most proximal oocyte had abnormal elongated shapes ( Figure 4 , C , D , and F , white outline ) and were not rectangular as the wild-type oocytes before maturation ( Figure 4A , white outline ) ( McCarter et al . , 1999 ) ." | Paper_evidence | WBPaper00036076 | ||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Caused_by_gene | WBGene00007626 | Paper_evidence | WBPaper00036076 | ||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| WBPhenotype:0001278 | Paper_evidence | WBPaper00036076 | |||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Remark | "PAT-3 : : GFP levels are high in the ER of the gonad DTCs at this stage of development , whereas fluorescence intensity decreased after induction of RNF-121 . The mean ratio of fluorescence intensity DTC / muscle ( DTC values were normalized to the fluorescence intensity in the muscle perinuclear region ; see Materials and Methods ) was lower in the PAT-3 : : GFP ;hsp-16p : : RNF-121 worms than in the PAT-3 : : GFP control strain ( 0.39 +/- 0.07 vs . 0.58 +/- 0.11 , respectively ; n = 50 for each group ; p < 0.0001 ) ( Figure 5 , A and B ) ." | Paper_evidence | WBPaper00036076 | ||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Caused_by_gene | WBGene00007626 | Paper_evidence | WBPaper00036076 | ||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| EQ_annotations | Anatomy_term | WBbt:0006865 | PATO:0000460 | Paper_evidence | WBPaper00036076 | ||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Phenotype_assay | Genotype | PAT-3 : : GFP | Paper_evidence | WBPaper00036076 | |||||
| Curator_confirmed | WBPerson2987 | ||||||||
| WBPhenotype:0001944 | Paper_evidence | WBPaper00036076 | |||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Remark | " After induction of RNF-121 at the mid-L2 stage , the adult gonad arms exhibited defective gametogenesis that resulted in proximal gonads with a small number of oocytes ( Figure 4 , C-H , 3.6 +/- 1.5 oocytes per gonad arm , n = 61 ) in mid- and late-stage adults compared with 9.6 +/- 1.5 oocytes in mid- stage wild-type adults ( n = 49 ) and 9.2 +/- 1.3 in mid-stage adults expressing a ligase-inactive RNF-121 mutant ( hsp-16 : : RNF-121 C222A C225A ;n = 67 ) ." | Paper_evidence | WBPaper00036076 | ||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Caused_by_gene | WBGene00007626 | Paper_evidence | WBPaper00036076 | ||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Used_for | Interactor | WBInteraction000520598 | |||||||
| Reference | WBPaper00036076 | ||||||||
| Species | Caenorhabditis elegans |
