WormBase Tree Display for Transgene: WBTransgene00006402
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| WBTransgene00006402 | Public_name | syEx125 | |
|---|---|---|---|
| Summary | [egl-30] | ||
| Synonym | [pMH86pLB2pBS] | ||
| Construction | Construct | WBCnstr00006250 | |
| Construction_summary | syEx125 is a multicopy extra-chromosomal array overexpressing the wild-type egl- 30 gene resulting in Gq alpha gain of function. A fragment of the Gq alpha gene egl-30 was amplified from C. elegans genomic DNA (Sulston and Hodgkin, 1988) by PCR using the degenerate primers, and was used for hybridization to identify a cDNA clone pLB1 (Barstead and Waterston, 1989) that was sequenced. pLB1 was used to identify a genomic lambda clone (Coulson et al., 1986), which was mapped to cosmid C57H9 and subcloned as a 10.5 kb XbaI fragment into Bluescript SK- to create pLB2. The positions of introns were determined by comparing sequence from coding region of the Gq alpha in pLB1 and pLB2. Their sizes were determined by PCR or sequencing. Two transgenic lines, syEx125 and syEx126, expressing egl-30 were generated by injection of pLB2 at 5 ng/ul with plasmid pMH86 at 16 ng/ul into dpy-20(e1282), as described by Mendel et al. (1995) (Brundage et al. 1996). | ||
| Genetic_information | Extrachromosomal | ||
| Reference | WBPaper00002466 | ||
| WBPaper00003193 | |||
| WBPaper00006314 | |||
| WBPaper00017508 | |||
| WBPaper00024552 | |||
| Species | Caenorhabditis elegans |
