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WormBase Tree Display for Transgene: WBTransgene00006333

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Name Class

WBTransgene00006333Public_nameatEx32
Summary[pCG9-2(nhr-25::gfp)]
ConstructionConstructWBCnstr00006181
Construction_summaryThe translational fusion, pCG9-2 is an independent isolate of pCG9 a construct that should permit utilization of both 5' ends of nhr-25::gfp. pCG9 was generated in the vector pPD95.67 (gift from A. Fire) by a PCR protocol (Cassata et al., 1998) employing the internal primer MK-1 (5'-[CCAATCCCGGGGATCCTCTAG]GCGTTT- GAAGAAGCCCTGTA-3'; GFP vector sequences bracketed) and the upstream primer MK-2 (5'-[CTTATCGCATGC]AGATGGCA- GAAGTGGGTGAC-3'; bracketed sequences provide a SphI restriction site).
Genetic_informationExtrachromosomal
ReferenceWBPaper00004124
WBPaper00004742
SpeciesCaenorhabditis elegans