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WormBase Tree Display for Transgene: WBTransgene00005238

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Name Class

WBTransgene00005238Public_namezuIs145
Summary[unc-119(+) + nmy-2::MOM-5::GFP]
Synonym[nmy-2::mom-5::gfp]
ConstructionConstructWBCnstr00005215
Integration_methodGamma_irradiation
Construction_summaryA full-length mom-5 cDNA was isolated from adultC. elegans RNA by RT-PCR and fused to the nmy-2 promoter and to the gene encoding GFP as follows: nmy-2/linker/5$(B!l(B mom-5: gcggtaataatgggcgcgccacatcgacat and 3$(B!l(B mom-5/linker/gfp: aatatgaggggaggaagtggtcctgcaggaggtatgagtaaa. Strains expressing MOM-5::GFP were obtained by microparticle bombardment of unc-119 worms and extrachromosomal arrays were integrated by gamma-irradiation.
Genetic_informationIntegrated
Used_forExpr_patternExpr12892
Associated_withStrainWBStrain00022504
ReferenceWBPaper00024672
WBPaper00046716
WBPaper00055870
WBPaper00059401
SpeciesCaenorhabditis elegans