WormBase Tree Display for Transgene: WBTransgene00004995
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| WBTransgene00004995 | Public_name | WBPaper00031252Is1 | |
|---|---|---|---|
| Summary | [ain-2::ain-2-gfp] | ||
| Synonym | [AIN-2::GFP] | ||
| Construction | Construct | WBCnstr00004972 | |
| Integration_method | Particle_bombardment | ||
| Laboratory | MH | ||
| Construction_summary | For the ain-2::gfp fusion gene, a Not I site was created just before ain-2's stop codon by site-directed mutagenesis on ain-2 genomic DNA. The GFP coding region was PCR amplified from ppD95.75 and inserted in-frame into the Not I site of the ain-2 gene. A single/low copy integration line of this construct was obtained by biolistic transformation. Quantitive-PCR analysis confirmed that only 1 or 2 copies of the AIN-2::GFP fusion gene was integrated. | ||
| full length functional translational fusion. | |||
| Genetic_information | Integrated | ||
| Used_for | Expr_pattern | Expr7851 | |
| Reference | WBPaper00031252 | ||
| Species | Caenorhabditis elegans |
