WormBase Tree Display for Transgene: WBTransgene00004864
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| WBTransgene00004864 | Public_name | vaIs9 | |
|---|---|---|---|
| Summary | [unc-2::gfp] | ||
| Construction | Construct | WBCnstr00004845 | |
| Construction_summary | Long-range PCR was used to amplify the 5' region of the unc-2 gene and the predicted first three exons. Two fragments were amplified from genomic DNA. One included ~4 kb of 5' sequence upstream from the first exon, along with a 6.8 kb region encompassing exons one and two. The second long-range PCR product was 4.1 kb in length, overlapping with the first product by 694 bp and ending within exon three. The second product was cloned into the vector pGEM-T easy. A green fluorescent protein (GFP) cassette isolated from the expression vector pPD95.70 was then cloned in-frame with exon 3, generating the construct punc24.70. The 10.8 kb long-range PCR product was injected into the gonads of adult lin-15(n765ts) hermaphrodites along with punc24.70 and pJM23, which contains a wild-type copy of the lin-15 gene. | ||
| Genetic_information | Integrated | ||
| Used_for | Expr_pattern | Expr2616 | |
| Associated_with | Strain | WBStrain00035025 | |
| Reference | WBPaper00006005 | ||
| Species | Caenorhabditis elegans |
