WormBase Tree Display for Transgene: WBTransgene00004805
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| WBTransgene00004805 | Public_name | ubIs5 | |
|---|---|---|---|
| Summary | [hsp16.1::hsp-16A::lacZ + rol-6(su1006)] | ||
| Synonym | [hsp16::lacZ + rol-6] | ||
| ubIn5 | |||
| PC72ubln5 | |||
| 48.1C | |||
| hsp16-lacZ | |||
| Construction | Construct | WBCnstr00019264 | |
| Coinjection | WBCnstr00004720 | ||
| Integration_method | Gamma_irradiation | ||
| Laboratory | PC | ||
| Construction_summary | Integrated hsp16/lacZ construct also described as strain PC72ubln5, which is an integrated array of 48.1C and pRF4 and was derived from PC11ubEx11 by gamma irradiation mutagenesis. The PC72 strain contains approximately 70-80 integrated copies of the transgene per haploid genome. beta-Galactosidase produced by PC72 nematodes is sequestered in cell nuclei due to the inclusion of an SV40 nuclear localization signal at the amino terminus of the enzyme, simplifying the identification of cells expressing the transgene. | ||
| Genetic_information | Integrated | ||
| Used_for | Interactor | WBInteraction000503900 | |
| Associated_with | Strain | WBStrain00030548 | |
| Reference (11) | |||
| Species | Caenorhabditis elegans |
