WormBase Tree Display for Transgene: WBTransgene00001851
expand all nodes | collapse all nodes | view schema
WBTransgene00001851 | Public_name | WBPaper00002501_In2 | |
---|---|---|---|
Summary | [Pubc-2::UBC-2::lacZ; pRF4] | ||
Synonym | pZMI.1In2 | ||
[LET-70::GFP] | |||
Construction | Construct | WBCnstr00001832 | |
Coinjection | WBCnstr00004720 | ||
Integration_method | Gamma_irradiation | ||
Laboratory | PC | ||
Construction_summary | Clone: pZMI.1 | ||
ubc-2 = let-70 --WS59. For this construct, the lacZ coding region, a 3.3kb BamHI-ApaI fragment from pPD16.43, was inserted in-frame into the second exon (BglII-ApaI sites) of the ubc-2 coding sequence, including five exons and four introns. A 6kb BglII fragment preceding the initiation methionine codon and 2.6kb of sequence downstream of the TAG stop codon were included in the construct to provide regulatory elements for expression. | |||
Genetic_information | Integrated | ||
Used_for | Expr_pattern | Expr725 | |
Reference | WBPaper00002501 | ||
Species | Caenorhabditis elegans |