WormBase Tree Display for Transgene: WBTransgene00001850
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| WBTransgene00001850 | Public_name | WBPaper00002501_In1 | |
|---|---|---|---|
| Summary | [Pubc-2::UBC-2::lacZ; pRF4] | ||
| Synonym | pZMI.1In1 | ||
| [LET-70::GFP] | |||
| Construction | Construct | WBCnstr00001832 | |
| Coinjection | WBCnstr00004720 | ||
| Integration_method | Gamma_irradiation | ||
| Laboratory | PC | ||
| Construction_summary | Clone: pZMI.1 | ||
| ubc-2 = let-70 --WS59. For this construct, the lacZ coding region, a 3.3kb BamHI-ApaI fragment from pPD16.43, was inserted in-frame into the second exon (BglII-ApaI sites) of the ubc-2 coding sequence, including five exons and four introns. A 6kb BglII fragment preceding the initiation methionine codon and 2.6kb of sequence downstream of the TAG stop codon were included in the construct to provide regulatory elements for expression. | |||
| Genetic_information | Integrated | ||
| Used_for | Expr_pattern | Expr725 | |
| Reference | WBPaper00002501 | ||
| Species | Caenorhabditis elegans |
