WormBase Tree Display for Transgene: WBTransgene00001847
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| WBTransgene00001847 | Public_name | pxIs7 | |
|---|---|---|---|
| Summary | [Ppha-4::CAAX-GFP; pRF4] | ||
| Synonym | GFP-PM | ||
| [pha-4p::pm::gfp, rol-6p:: rol-6(su1006)] | |||
| Construction | Construct | WBCnstr00001829 | |
| Coinjection | WBCnstr00004720 | ||
| Laboratory | SM | ||
| Construction_summary | GFP-PM, targets GFP to the plasma membrane using the isoprenylation sequence of mig-2 (Zipkin et al., 1997). Construct was microinjected into C. elegans by using the following injection mix: 20 ng/ul GFP construct, 100 ng/ul sheared herring sperm genomic DNA, 30 ng/ul 1-kb ladder (Gibco BRL), and 100 ng/ul pRF4. | ||
| Genetic_information | Integrated | ||
| Associated_with | Strain | WBStrain00045497 | |
| Reference | WBPaper00004707 | ||
| WBPaper00042427 | |||
| WBPaper00046712 | |||
| Species | Caenorhabditis elegans | ||
| Remark | Published as pIs7 in Budirahardja et al., 2015 (WBPaper00046712) and Paitis et al., 2013 (WBPaper42427). |
