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WormBase Tree Display for Transgene: WBTransgene00001846

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Name Class

WBTransgene00001846Public_namepxIs6
Summary[Ppha-4::GFP::HIS-11]
SynonymGFP-N
[pha-4::gfp:h2b; rol-6(su1006)]
[pha-4::gfp::HIS11]
ConstructionConstructWBCnstr00001828
CoinjectionWBCnstr00004720
LaboratorySM
Construction_summaryGFP-N, localizes GFP to the nucleus with a fusion tohis-11, a histone 2B homologue (Seydoux, personal communication; J. Waddle, personal communication). Construct was microinjected into C. elegans by using the following injection mix: 20 ng/ul GFP construct, 100 ng/ul sheared herring sperm genomic DNA, 30 ng/ul 1-kb ladder (Gibco BRL), and 100 ng/ul pRF4.
Genetic_informationIntegrated
Associated_withStrainWBStrain00034106
ReferenceWBPaper00004707
WBPaper00005115
WBPaper00005518
WBPaper00024980
WBPaper00035290
WBPaper00036156
WBPaper00041027
WBPaper00042427
WBPaper00051293
SpeciesCaenorhabditis elegans
RemarkPublished as pIs6 in Paitis et al., 2013 (WBPaper42427).