WormBase Tree Display for Transgene: WBTransgene00001633
expand all nodes | collapse all nodes | view schema
WBTransgene00001633 | Public_name | oxEx839 | |
---|---|---|---|
Summary | [unc-80::gfp] | ||
Construction | Construct | WBCnstr00001622 | |
Construction_summary | The unc-80 promoter was amplified from genomic DNA with the following primers: U80A2 5' -GATATCCGCATATTGAGAGCTTTAG-3' and U80B 5 -CCTTTACTCATTTTTTCTACCGGTACcaatggcactgaatcacattcttc-3'. GFP sequence was amplified with primers U80C 5' -GTACCGGTAGAAAAAATGAGTAAAGG-3' and U80D 5' -AAGGGCCCGTACGGCCGACTAGTAGG-3'. The two fragments were mixed together and then PCR was performed with the outside primers, U80A and U80D. The PCR fragment was injected into lin-15(n765ts) mutants at 5 ng/ml. Three lines were obtained that had similar expression, and the analyzed array is oxEx839. --precise ends. | ||
Genetic_information | Extrachromosomal | ||
Used_for | Expr_pattern | Expr4994 | |
Reference | WBPaper00031000 | ||
Species | Caenorhabditis elegans |