WormBase Tree Display for Transgene: WBTransgene00001631
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| WBTransgene00001631 | Public_name | oxEx469 | |
|---|---|---|---|
| Summary | [exp-1::exp-1-gfp] | ||
| Construction | Construct | WBCnstr00001620 | |
| Construction_summary | translational fusion. GFP was inserted in-frame within the EXP-1 open reading frame. The GFP coding region from pPD95.75 was amplified with primers tagged with ApaI sites on both the 5 and 3 ends. This PCR product was gel-purified and inserted in frame into an ApaI site of pAB03 to generate pAB05. This fusion protein contains GFP in the intracellular loop between M3 and M4. This translational exp-1:GFP reporter was coinjected at 1 ng/l with pEK1 at 30 ng/l and 69 ng/l of 1-kb DNA Ladder (Gibco) into exp-1(sa6); lin-15(n765ts) animals. The stable transgenic lines (oxEx469) were rescued for enteric muscle contractions and showed GFP fluorescence in the enteric muscles. | ||
| Genetic_information | Extrachromosomal | ||
| Used_for | Expr_pattern | Expr2760 | |
| Reference | WBPaper00006173 | ||
| Species | Caenorhabditis elegans |
