WormBase Tree Display for Transgene: WBTransgene00001047
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WBTransgene00001047 | Public_name | muIs53 | ||||||
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Summary | [hsp-16.2::egl-20] | |||||||
Construction | Construct | WBCnstr00001038 | ||||||
Construction_summary | The hs-egl-20 fusion (pJW30) was generated by inserting a PCR-amplified egl-20 cDNA fragment with an engineered stop codon into NheI-SacI digested pPD49.78 (hsp 162, from A. Fire). The egl-20 cDNA was amplified using the primer pair JW28 (5 -CCGGGGCCCGCTAGCATGCAATTTTTCATTTGC-3 ) and JW29 (5 -CCGGAGCTCCTGCAGTTTGCATGTATGTACTGC-3 ). muIs53 was made by integrating an extrachromosomal array containing pJW30 injected at 20 ng/ul and pPD10.46 coinjected at 125 ng/ul. | |||||||
Genetic_information | Integrated | |||||||
Phenotype | WBPhenotype:0001235 | Paper_evidence | WBPaper00004436 | |||||
Curator_confirmed | WBPerson2987 | |||||||
Remark | Overexpression of egl-20 via a heat shock promoter and a 10 minute (or 30 minute) heat shock pulse resulted in aberrant V cell division polarities for all V cells, V1 through V6 (Figure 2C, Table 3) | Paper_evidence | WBPaper00004436 | |||||
Curator_confirmed | WBPerson2987 | |||||||
Caused_by_gene | WBGene00001188 | Paper_evidence | WBPaper00004436 | |||||
Curator_confirmed | WBPerson2987 | |||||||
EQ_annotations | Anatomy_term (12) | |||||||
Phenotype_assay | Treatment | Animals were heat shocked at 33 degrees Celsius and then recovered at 20 degrees Celsius. Polarity of the V cell divisions was determined after the V cell daughters had differentiated (approx. 8 hours following heat shock). | Paper_evidence | WBPaper00004436 | ||||
Curator_confirmed | WBPerson2987 | |||||||
Genotype | egl-20(n585) | Paper_evidence | WBPaper00004436 | |||||
Curator_confirmed | WBPerson2987 | |||||||
Used_for | Interactor | WBInteraction000524388 | ||||||
Associated_with | Strain | WBStrain00004842 | ||||||
Reference (11) | ||||||||
Species | Caenorhabditis elegans |