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WormBase Tree Display for Transgene: WBTransgene00001047

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Name Class

WBTransgene00001047Public_namemuIs53
Summary[hsp-16.2::egl-20]
ConstructionConstructWBCnstr00001038
Construction_summaryThe hs-egl-20 fusion (pJW30) was generated by inserting a PCR-amplified egl-20 cDNA fragment with an engineered stop codon into NheI-SacI digested pPD49.78 (hsp 162, from A. Fire). The egl-20 cDNA was amplified using the primer pair JW28 (5 -CCGGGGCCCGCTAGCATGCAATTTTTCATTTGC-3 ) and JW29 (5 -CCGGAGCTCCTGCAGTTTGCATGTATGTACTGC-3 ). muIs53 was made by integrating an extrachromosomal array containing pJW30 injected at 20 ng/ul and pPD10.46 coinjected at 125 ng/ul.
Genetic_informationIntegrated
PhenotypeWBPhenotype:0001235Paper_evidenceWBPaper00004436
Curator_confirmedWBPerson2987
RemarkOverexpression of egl-20 via a heat shock promoter and a 10 minute (or 30 minute) heat shock pulse resulted in aberrant V cell division polarities for all V cells, V1 through V6 (Figure 2C, Table 3)Paper_evidenceWBPaper00004436
Curator_confirmedWBPerson2987
Caused_by_geneWBGene00001188Paper_evidenceWBPaper00004436
Curator_confirmedWBPerson2987
EQ_annotationsAnatomy_term (12)
Phenotype_assayTreatmentAnimals were heat shocked at 33 degrees Celsius and then recovered at 20 degrees Celsius. Polarity of the V cell divisions was determined after the V cell daughters had differentiated (approx. 8 hours following heat shock).Paper_evidenceWBPaper00004436
Curator_confirmedWBPerson2987
Genotypeegl-20(n585)Paper_evidenceWBPaper00004436
Curator_confirmedWBPerson2987
Used_forInteractorWBInteraction000524388
Associated_withStrainWBStrain00004842
Reference (11)
SpeciesCaenorhabditis elegans