WormBase Tree Display for Transgene: WBTransgene00000775
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| WBTransgene00000775 | Public_name | kIn2-1 | |
|---|---|---|---|
| Summary | [unc-18(+); unc-18(e81); rol-6(su1006)] | ||
| Construction | Construct | WBCnstr00000770 | |
| Coinjection | WBCnstr00004720 | ||
| Integration_method | Gamma_irradiation | ||
| Construction_summary | DNA construct punc-18 plasmid (20 ug/ml) was injected into unc-18(e81) by standard germline transformation techniques. pRF4 plasmid (50 ug/ml) containing the dominant roller marker rol-6(su1006) was co-injected to identify transgenic animals. The chromosomal integrations of extrachromosomal arrays were performed by gamma ray irradiation. Two independent integrated lines (kIn2-1 and kIn2--2) were identified from the progenies of 15 kEx2 animals that had been treated with 3780 rad from a 137Cs source. | ||
| Genetic_information | Integrated | ||
| Reference | WBPaper00002587 | ||
| Species | Caenorhabditis elegans |
