WormBase Tree Display for Transgene: WBTransgene00000754
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| WBTransgene00000754 | Public_name | kaIs12 | |||
|---|---|---|---|---|---|
| Summary | [COL-19::GFP] | ||||
| Synonym | [col-19::gfp] | ||||
| Construction | Construct | WBCnstr00000750 | |||
| Integration_method | Gamma_irradiation | ||||
| Construction_summary | The promoter and the full-length coding sequences col-19 were cloned in-frame to the green fluorescent protein GFP-C3 in a pSP65 backbone. The following primers were used to generate products with compatible restriction sites to the GFP expression vector: col19-A 5 -cgcctgcagCATTTGAAAATTTGCACC-3 (PstI) and col19-B 5 -ggggtaccGCCTTGTAAGCTGCACG-3 (KpnI). Amplified genomic products were digested and ligated into similarly cut vectors. Collagen COL-19 with GFP fused to C-terminus localised in the cuticle. --precise ends. | ||||
| Genetic_information | Integrated | ||||
| Phenotype | WBPhenotype:0000138 | Paper_evidence | WBPaper00035868 | ||
| Curator_confirmed | WBPerson426 | ||||
| WBPhenotype:0000948 | Paper_evidence | WBPaper00035868 | |||
| Curator_confirmed | WBPerson426 | ||||
| WBPhenotype:0001211 | Paper_evidence | WBPaper00035868 | |||
| Curator_confirmed | WBPerson426 | ||||
| Used_for | Expr_pattern | Expr15674 | |||
| Interactor (33) | |||||
| Associated_with | Strain | WBStrain00034928 | |||
| Reference (23) | |||||
| Species | Caenorhabditis elegans |
