WormBase Tree Display for Transgene: WBTransgene00000657
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| WBTransgene00000657 | Public_name | WBPaper00006024Is1 | |
|---|---|---|---|
| Summary | [Pskn-1::SKN-1::gfp] | ||
| Synonym | Is001 | ||
| Construction | Construct | WBCnstr00000655 | |
| Integration_method | UV | ||
| Construction_summary | Integrated from Ex001. [skn-1::gfp] translational fusion. skn-1 gfp promoter fusion construct (SknPro GFP) was created by ligating GFP vector pPD95.67 and a PCR-amplified 2.1-kb clone containing the promoter region and 38 amino acids from the first ATG codon of the skn-1 gene from cosmid T19E7. To generate the SKN-1 GFP translational fusion construct, the 5.7-kb EcoRI DNA fragment that rescues the maternal skn-1 phenotype and encodes the 533-amino-acid SKN-1 protein was amplified from cosmid B0547. A ClaI site was created immediately 3' to the SKN-1 C terminus by the QuikChange method (Stratagene), which was used for all site-directed mutagenesis. This EcoRI fragment was subcloned into pUC18, which contained the upstream 1.3-kb SphIEcoRI fragment from SknPro GFP. A 0.8-kb ClaI fragment that contained the GFP open reading frame (amplified from plasmid pPD114.35) was then cloned into the ClaI site to generate an in-frame exon fusion of GFP to the SKN-1 C terminus. | ||
| Genetic_information | Integrated | ||
| Used_for | Expr_pattern | Expr2646 | |
| Interactor | WBInteraction000518425 | ||
| Reference | WBPaper00006024 | ||
| Species | Caenorhabditis elegans |
