WormBase Tree Display for Transgene: WBTransgene00000637
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| WBTransgene00000637 | Public_name | idIs5 | |
|---|---|---|---|
| Summary | [fem-1::lacz] | ||
| Construction | Construct | WBCnstr00000635 | |
| Integration_method | Gamma_irradiation | ||
| Construction_summary | [fem-1::lacZ] translational fusion. A BamHI site was introduced at position +3 of fem-1 by mutagenesis with oligonucleotides oAS140 (5ATGTCCATTTGGATCCATACCGCCA-3). A BglII-BamHI fragment extending 1.9 kb upstream from BamHI site of lacZ fusion vector pPD16.43, placing ATG codon in frame with lacZ coding sequence. A 5.9 kb EcoRV-SpeI fragment extending from position +202 in exon 2 of the fem-1 gene to position +6095, 1.43 kb 3 to the site of polyadenylation of fem-1 mRNA was ligate between StuI and SpeI sites downstream of lacZ stop codon. AS#1209 contains all sequences present in genomic DNA fragment capable of rescuing fem-1 mutant except for a 200-bp region extending from within exon1 and 2, that region is replaced by lacZ. --precise ends. | ||
| Genetic_information | Integrated | ||
| Map | V | ||
| Used_for | Expr_pattern | Expr674 | |
| Reference | WBPaper00002504 | ||
| Species | Caenorhabditis elegans |
