WormBase Tree Display for Transgene: WBTransgene00000618
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| WBTransgene00000618 | Public_name | huIs29 | |||||||
|---|---|---|---|---|---|---|---|---|---|
| Summary | [mec-7::gfp; bar-1 genomic sequence; dpy-20 genomic sequence] | ||||||||
| Construction | Construct | WBCnstr00000616 | |||||||
| Integration_method | Gamma_irradiation | ||||||||
| Construction_summary | Clone = pMH86 (dpy-20 genomic sequence). | ||||||||
| Clone = pDE204 (bar-1 genomic sequence). | |||||||||
| Clone = pPD117.01 (a mec-7::gfp fusion construct). | |||||||||
| Genetic_information | Integrated | ||||||||
| Phenotype | WBPhenotype:0000012 | Paper_evidence | WBPaper00025199 | ||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Remark | "Overexpression of BAR-1 enhanced the daf-2-induced formation of dauer larvae (Fig. 1A)." | Paper_evidence | WBPaper00025199 | ||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Caused_by_gene | WBGene00000238 | Paper_evidence | WBPaper00025199 | ||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| EQ_annotations | Life_stage | WBls:0000032 | PATO:0000460 | Paper_evidence | WBPaper00025199 | ||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Phenotype_assay | Temperature | 20 | Paper_evidence | WBPaper00025199 | |||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Genotype | daf-2(m41); bar-1(ga80) | Paper_evidence | WBPaper00025199 | ||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| WBPhenotype:0000461 | Paper_evidence | WBPaper00025199 | |||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Remark | "Furthermore, overexpression of BAR-1 resulted in resistance to oxidative stress." (Figure 4C) | Paper_evidence | WBPaper00025199 | ||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Caused_by_gene | WBGene00000238 | Paper_evidence | WBPaper00025199 | ||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Affected_by | Molecule | WBMol:00002747 | Paper_evidence | WBPaper00025199 | |||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Phenotype_assay | Treatment | Animals were exposed to increasing concentrations of paraquat (0.2 mM, 0.3 mM, and 0.4 mM) and assessed for survival | Paper_evidence | WBPaper00025199 | |||||
| Curator_confirmed | WBPerson2987 | ||||||||
| WBPhenotype:0001236 | Paper_evidence | WBPaper00025199 | |||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Remark | "Indeed, overexpression of BAR-1 (in a wild-type bar-1 background) led to a modest, daf-16-dependent increase in sod-3 expression (Fig. 4B; fig. S2)." | Paper_evidence | WBPaper00025199 | ||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Caused_by_gene | WBGene00000238 | Paper_evidence | WBPaper00025199 | ||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Affected_by | Molecule | WBMol:00002747 | Paper_evidence | WBPaper00025199 | |||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Phenotype_assay | Treatment | Animals were exposed to 0.25 mM Paraquat | Paper_evidence | WBPaper00025199 | |||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Genotype | huIs33 [sod-3::gfp] | Paper_evidence | WBPaper00025199 | ||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Reference | WBPaper00025199 | ||||||||
| Species | Caenorhabditis elegans |
