WormBase Tree Display for Transgene: WBTransgene00000415
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WBTransgene00000415 | Public_name | dvIs27 | ||||||
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Summary | [pAF29(myo-3p::human Aβ 1-42::let-851 3'UTR) + pRF4(rol-6(su1006))] | |||||||
Synonym | [myo-3::Aβ(1 to 42)-let 3'UTR(pAF29); pRF4 (rol-6(su1006)] | |||||||
[pAF29 (Pmyo-3::Aβ42); pRF4] | ||||||||
Construction | Construct | WBCnstr00000415 | ||||||
Coinjection | WBCnstr00004720 | |||||||
Genetic_information | Integrated | |||||||
Map | X | |||||||
Phenotype | WBPhenotype:0000019 | Paper_evidence | WBPaper00050306 | |||||
Curator_confirmed | WBPerson712 | |||||||
Remark | Strain CL4176 accumulates amyloid-beta 1-42 in body wall muscles after being shifted from 15 to 25 deg C during L4 (Link et al. 2003). Forty-eight hours after a temperature shift, control CL802 worms showed normal pumping activity (A) during microfluidic electropharyngeogram (EPG) recordings in M9 buffer containing 10 mM serotonin (Lockery et al. 2012). In contrast, mean pump frequency in CL4176 worms was reduced significantly by ~42% (A. CL802, 3.70 +/- 0.15 Hz; CL4176, 2.16 +/- 0.23 Hz; S.E.M.; P <10-4; 2-tailed Student's t-test), due to a marked increase in the probability of longer inter-pump intervals (the time between successive pumps B). Reviewed by C. Link and N. Liachko. | Paper_evidence | WBPaper00050306 | |||||
Curator_confirmed | WBPerson712 | |||||||
Image | WBPicture0000013532 | Paper_evidence | WBPaper00050306 | |||||
Curator_confirmed | WBPerson712 | |||||||
Caused_by_other | human amyloid beta 1-42 | Paper_evidence | WBPaper00050306 | |||||
Curator_confirmed | WBPerson712 | |||||||
Affected_by | Molecule | WBMol:00004929 | Paper_evidence | WBPaper00050306 | ||||
Curator_confirmed | WBPerson712 | |||||||
Phenotype_assay | Strain | WBStrain00005113 | Paper_evidence | WBPaper00050306 | ||||
Curator_confirmed | WBPerson712 | |||||||
Control_strain | WBStrain00005062 | Paper_evidence | WBPaper00050306 | |||||
Curator_confirmed | WBPerson712 | |||||||
WBPhenotype:0000644 | Paper_evidence | WBPaper00037063 | ||||||
Curator_confirmed | WBPerson712 | |||||||
Remark | Induction of expression of Human beta amyloid protein Ab42 leads to a highly reproducible paralysis phenotype, in which all induced animals become paralyzed within 28 hr of temperature upshift. | Paper_evidence | WBPaper00037063 | |||||
Curator_confirmed | WBPerson712 | |||||||
Caused_by_other | Abeta42 Beta amyloid peptide | Paper_evidence | WBPaper00037063 | |||||
Curator_confirmed | WBPerson712 | |||||||
WBPhenotype:0000962 | Paper_evidence | WBPaper00037063 | ||||||
Curator_confirmed | WBPerson712 | |||||||
Remark | No gene induction was observed for general heat shock response (Phsp-16::GFP), the unfolded protein response (Phsp-4::GFP), or reduced insulin-like signaling (nuclear localization of daf-16::GFP)(data not shown). However, significant induction was observed for a Pgst-4::GFP reporter. | Paper_evidence | WBPaper00037063 | |||||
Curator_confirmed | WBPerson712 | |||||||
Caused_by_other | Abeta42 Beta amyloid peptide | Paper_evidence | WBPaper00037063 | |||||
Curator_confirmed | WBPerson712 | |||||||
WBPhenotype:0001703 | Paper_evidence | WBPaper00049135 | ||||||
Curator_confirmed | WBPerson18325 | |||||||
Remark | Fig. 6 A and B show the protective effect of A1-6A2V-TAT(D) peptide on the reduction of body bends induced in CL2120 transgenic worms by A. | Paper_evidence | WBPaper00049135 | |||||
Curator_confirmed | WBPerson18325 | |||||||
Caused_by_other | Amyloid Beta | Paper_evidence | WBPaper00049135 | |||||
Curator_confirmed | WBPerson18325 | |||||||
WBPhenotype:0001935 | Paper_evidence | WBPaper00049135 | ||||||
Curator_confirmed | WBPerson18325 | |||||||
Remark | Fig. 3 shows the ability of Aβ1-6A2V-TAT(D) peptide to protect CL4176 transgenic worms against oligomeric Aβ1-42 induced paralysis. | Paper_evidence | WBPaper00049135 | |||||
Curator_confirmed | WBPerson18325 | |||||||
Caused_by_other | Amyloid Beta | Paper_evidence | WBPaper00049135 | |||||
Curator_confirmed | WBPerson18325 | |||||||
WBPhenotype:0002441 | Paper_evidence | WBPaper00050306 | ||||||
Curator_confirmed | WBPerson712 | |||||||
Remark | Strain CL4176 accumulates amyloid-beta 1-42 in body wall muscles after being shifted from 15 to 25 deg C during L4 (Link et al. 2003). Forty-eight hours after a temperature shift, control CL802 worms showed normal pumping activity (A) during microfluidic electropharyngeogram (EPG) recordings in M9 buffer containing 10 mM serotonin (Lockery et al. 2012). In contrast, mean pump frequency in CL4176 worms was reduced significantly by ~42% (A. CL802, 3.70 +/- 0.15 Hz; CL4176, 2.16 +/- 0.23 Hz; S.E.M.; P <10-4; 2-tailed Student's t-test), due to a marked increase in the probability of longer inter-pump intervals (the time between successive pumps B). Reviewed by C. Link and N. Liachko. | Paper_evidence | WBPaper00050306 | |||||
Curator_confirmed | WBPerson712 | |||||||
Image | WBPicture0000013532 | Paper_evidence | WBPaper00050306 | |||||
Curator_confirmed | WBPerson712 | |||||||
Caused_by_other | human amyloid beta 1-42 | Paper_evidence | WBPaper00050306 | |||||
Curator_confirmed | WBPerson712 | |||||||
Affected_by | Molecule | WBMol:00004929 | Paper_evidence | WBPaper00050306 | ||||
Curator_confirmed | WBPerson712 | |||||||
Phenotype_assay | Strain | WBStrain00005113 | Paper_evidence | WBPaper00050306 | ||||
Curator_confirmed | WBPerson712 | |||||||
Control_strain | WBStrain00005062 | Paper_evidence | WBPaper00050306 | |||||
Curator_confirmed | WBPerson712 | |||||||
Associated_with | Strain | WBStrain00005113 | ||||||
WBStrain00005115 | ||||||||
Reference (64) | ||||||||
Species | Caenorhabditis elegans |