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WormBase Tree Display for Transgene: WBTransgene00000415

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Name Class

WBTransgene00000415Public_namedvIs27
Summary[pAF29(myo-3p::human Aβ 1-42::let-851 3'UTR) + pRF4(rol-6(su1006))]
Synonym[myo-3::Aβ(1 to 42)-let 3'UTR(pAF29); pRF4 (rol-6(su1006)]
[pAF29 (Pmyo-3::Aβ42); pRF4]
ConstructionConstructWBCnstr00000415
CoinjectionWBCnstr00004720
Genetic_informationIntegrated
MapX
PhenotypeWBPhenotype:0000019Paper_evidenceWBPaper00050306
Curator_confirmedWBPerson712
RemarkStrain CL4176 accumulates amyloid-beta 1-42 in body wall muscles after being shifted from 15 to 25 deg C during L4 (Link et al. 2003). Forty-eight hours after a temperature shift, control CL802 worms showed normal pumping activity (A) during microfluidic electropharyngeogram (EPG) recordings in M9 buffer containing 10 mM serotonin (Lockery et al. 2012). In contrast, mean pump frequency in CL4176 worms was reduced significantly by ~42% (A. CL802, 3.70 +/- 0.15 Hz; CL4176, 2.16 +/- 0.23 Hz; S.E.M.; P <10-4; 2-tailed Student's t-test), due to a marked increase in the probability of longer inter-pump intervals (the time between successive pumps B). Reviewed by C. Link and N. Liachko.Paper_evidenceWBPaper00050306
Curator_confirmedWBPerson712
ImageWBPicture0000013532Paper_evidenceWBPaper00050306
Curator_confirmedWBPerson712
Caused_by_otherhuman amyloid beta 1-42Paper_evidenceWBPaper00050306
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00004929Paper_evidenceWBPaper00050306
Curator_confirmedWBPerson712
Phenotype_assayStrainWBStrain00005113Paper_evidenceWBPaper00050306
Curator_confirmedWBPerson712
Control_strainWBStrain00005062Paper_evidenceWBPaper00050306
Curator_confirmedWBPerson712
WBPhenotype:0000644Paper_evidenceWBPaper00037063
Curator_confirmedWBPerson712
RemarkInduction of expression of Human beta amyloid protein Ab42 leads to a highly reproducible paralysis phenotype, in which all induced animals become paralyzed within 28 hr of temperature upshift.Paper_evidenceWBPaper00037063
Curator_confirmedWBPerson712
Caused_by_otherAbeta42 Beta amyloid peptidePaper_evidenceWBPaper00037063
Curator_confirmedWBPerson712
WBPhenotype:0000962Paper_evidenceWBPaper00037063
Curator_confirmedWBPerson712
RemarkNo gene induction was observed for general heat shock response (Phsp-16::GFP), the unfolded protein response (Phsp-4::GFP), or reduced insulin-like signaling (nuclear localization of daf-16::GFP)(data not shown). However, significant induction was observed for a Pgst-4::GFP reporter.Paper_evidenceWBPaper00037063
Curator_confirmedWBPerson712
Caused_by_otherAbeta42 Beta amyloid peptidePaper_evidenceWBPaper00037063
Curator_confirmedWBPerson712
WBPhenotype:0001703Paper_evidenceWBPaper00049135
Curator_confirmedWBPerson18325
RemarkFig. 6 A and B show the protective effect of A1-6A2V-TAT(D) peptide on the reduction of body bends induced in CL2120 transgenic worms by A.Paper_evidenceWBPaper00049135
Curator_confirmedWBPerson18325
Caused_by_otherAmyloid BetaPaper_evidenceWBPaper00049135
Curator_confirmedWBPerson18325
WBPhenotype:0001935Paper_evidenceWBPaper00049135
Curator_confirmedWBPerson18325
RemarkFig. 3 shows the ability of A&beta;1-6A2V-TAT(D) peptide to protect CL4176 transgenic worms against oligomeric A&beta;1-42 induced paralysis.Paper_evidenceWBPaper00049135
Curator_confirmedWBPerson18325
Caused_by_otherAmyloid BetaPaper_evidenceWBPaper00049135
Curator_confirmedWBPerson18325
WBPhenotype:0002441Paper_evidenceWBPaper00050306
Curator_confirmedWBPerson712
RemarkStrain CL4176 accumulates amyloid-beta 1-42 in body wall muscles after being shifted from 15 to 25 deg C during L4 (Link et al. 2003). Forty-eight hours after a temperature shift, control CL802 worms showed normal pumping activity (A) during microfluidic electropharyngeogram (EPG) recordings in M9 buffer containing 10 mM serotonin (Lockery et al. 2012). In contrast, mean pump frequency in CL4176 worms was reduced significantly by ~42% (A. CL802, 3.70 +/- 0.15 Hz; CL4176, 2.16 +/- 0.23 Hz; S.E.M.; P <10-4; 2-tailed Student's t-test), due to a marked increase in the probability of longer inter-pump intervals (the time between successive pumps B). Reviewed by C. Link and N. Liachko.Paper_evidenceWBPaper00050306
Curator_confirmedWBPerson712
ImageWBPicture0000013532Paper_evidenceWBPaper00050306
Curator_confirmedWBPerson712
Caused_by_otherhuman amyloid beta 1-42Paper_evidenceWBPaper00050306
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00004929Paper_evidenceWBPaper00050306
Curator_confirmedWBPerson712
Phenotype_assayStrainWBStrain00005113Paper_evidenceWBPaper00050306
Curator_confirmedWBPerson712
Control_strainWBStrain00005062Paper_evidenceWBPaper00050306
Curator_confirmedWBPerson712
Associated_withStrainWBStrain00005113
WBStrain00005115
Reference (64)
SpeciesCaenorhabditis elegans