WormBase Tree Display for Transgene: WBTransgene00000400
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WBTransgene00000400 | Public_name | dhIs64 | |
---|---|---|---|
Summary | [daf-9p::daf-9::GFP + lin-15(+)] | ||
Synonym | [daf-9::gfp] | ||
Construction | Construct | WBCnstr00000400 | |
Construction_summary | daf-9::gfp (10 ng/ul) and lin-15(+) marker (75 ng/ul) were injected into lin-15(n765) animals. dhEx61 and dhEx66 extrachromosomal lines fully rescued daf-9 Mig and Daf-c phenotypes. Integrants dhIs59 and dhIs64 were made from these as described. | ||
For daf-9::gfp construction, a 9.7 kb genomic fragment containing the daf-9 coding region and 7.4 kb upstream was amplified with primers 5'-GTAGTAATCT GTCCATTGGG GACTACTG-3' and 5'-GGGGTACCTT GATGAGACGA TTTCCGACC-3', and cloned into pCR-Blunt II-TOPO vector (Invitrogen). gfp coding region was then cloned into KpnI-digested daf-9-TOPO. | |||
translational fusion, a functional gene fusion that rescues mutant phenotypes. --precise ends. | |||
Genetic_information | Integrated | ||
Used_for | Expr_pattern (2) | ||
Interactor | WBInteraction000518465 | ||
WBInteraction000521999 | |||
Associated_with | Strain | WBStrain00000020 | |
Reference | WBPaper00005034 | ||
WBPaper00005054 | |||
WBPaper00013396 | |||
WBPaper00032252 | |||
WBPaper00040978 | |||
WBPaper00040979 | |||
WBPaper00044922 | |||
WBPaper00045403 | |||
WBPaper00059297 | |||
Species | Caenorhabditis elegans |