WormBase Tree Display for Transgene: WBTransgene00000216

WBTransgene00000216 Public_name: ccIs4251
  Summary: [myo-3p::GFP(NLS)::LacZ (pSAK2) + myo-3p::GFP (mitochondrially targeted) (pSAK4) + dpy-20(+)]
  Synonym: [(pSAK2) myo-3p::GFP-LacZ(NLS) + (pSAK4) myo-3p::mitochondrial GFP]
    [myo-3p::GFP(NLS)::LacZ (pSAK2) + myo-3p::GFP (mitochondrially targeted) (pSAK4) + dpy-20(+)]
    [myo-3p::GFP::lacZ::NLS + myo-3p::GFP]
    [myo-3p::gfp]
    [myo-3prom::DFP-LacZ(NLS); myo-3prom::mitochondrial GFP; dpy-20(+)]
    [pSAK2 (myo-3p::GFP-LacZ(NLS)) + pSAK4 (myo-3p::mitochondrial GFP) + dpy-20(+)]
    [pSAK2 (myo-3p::GFP-LacZ(NLS)); pSAK4 (myo-3p::mitochondrial GFP); dpy-20(+)]
    ccIn4251
  Construction: Construct: WBCnstr00019450
      WBCnstr00000216
    Coinjection: WBCnstr00019441
    Coinjection_other: dpy-20
  Construction_summary: Located at the center of Chromosome I. "...integrated array (ccIs4251) made up of three plasmids: pSAK4 (myo-3 promoter driving mitochondrially targeted GFP); pSAK2 (myo-3 promoter driving a nuclear targeted GFPLacZ fusion); and a dpy-20 subclone as a selectable marker." pSAK2(myo-3::NgfplacZ), pSAK4(myo-3::Mtgfp), and pMH86 (Fire et al. 1998. Nature391: 806811).. "The multicopy tandem-array character of the ccIs4251 transgene was confirmed by Southern blot analysis (showing 100 copies each of pSAK2 and pSAK4; data not shown). ccIs4251 was obtained following treatment with grays and shows some crossover suppression activity in the center of chromosome I."(Hsieh et al. 1999). "To balance mutations linked to LGI, we used unc-15(e73) with a closely linked GFP transgene (ccIs4251)" (Eckmann et al.2004)
  Genetic_information: Integrated
    Map: I
  Used_for: Expr_pattern: Chronogram1363
      Marker38
    Marker_for: muscle Paper_evidence: WBPaper00031556
          WBPaper00044254
          WBPaper00045133
      muscle mitochondrial structure Paper_evidence: WBPaper00031556
          WBPaper00044254
          WBPaper00045133
      body wall muscles Paper_evidence: WBPaper00031556
          WBPaper00044254
          WBPaper00045133
      vulval muscles Paper_evidence: WBPaper00031556
          WBPaper00044254
          WBPaper00045133
    Interactor: WBInteraction000501937
      WBInteraction000555946
      WBInteraction000555947
      WBInteraction000555949
      WBInteraction000555950
  Associated_with: Strain (74)
  Reference (152)
  Species: Caenorhabditis elegans