WormBase Tree Display for Transgene: WBTransgene00000179
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| WBTransgene00000179 | Public_name | bxIs13 | |
|---|---|---|---|
| Summary | [egl-5::GFP + lin-15(+)] | ||
| Synonym | [egl-5::GFP] | ||
| [egl-5::gfp, lin-15] | |||
| Construction | Construct | WBCnstr00000179 | |
| Construction_summary | EM#286 was constructed by introducing restriction sites into PCR primers. About 7 kb 5' region, coding region, and 3'UTR of egl-5 was cloned into pBluescript (Stratagene). GFP (pPD102.33) was inserted near the C terminal of EGL-5. This reporter covers cosmid C08C3 nucleotides 32,429 to 43,981 and includes 7046 nucleotides of upstream sequence. It failed to rescue an egl-5 mutant despite the intention to make it a functional reporter. EM#286 was coinjected with pJM23 into strain him-5(e1490); lin-15(n765). The extrachromosomal array was subsequently integrated to generate bxIs13. | ||
| Genetic_information | Integrated | ||
| Map | X | ||
| Used_for | Expr_pattern | Expr11967 | |
| Interactor (13) | |||
| Associated_with | Strain | WBStrain00007187 | |
| WBStrain00008581 | |||
| WBStrain00008584 | |||
| Reference (16) | |||
| Species | Caenorhabditis elegans |
