WormBase Tree Display for Transgene: WBTransgene00000128
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| WBTransgene00000128 | Public_name | baIn8 | |
|---|---|---|---|
| Summary | [unc-54::Q82-GFP; tor-2(D368)(+)] | ||
| Construction | Construct | WBCnstr00000128 | |
| Integration_method | Gamma_irradiation | ||
| Construction_summary | tQ82-GFP is a translational fusion of 82 glutamines to GFP. To obtain the D368 mutant form of tor-2, an initial round of PCR was performed to generate an approximately 1 kb cDNA (corresponding to amino acids 1367 of TOR-2) using primers 1 and 3 (5'-GGGAAAAATTCAAGATCAAGAACTCTTTGCATG-3'). In parallel, an ~200 bp fragment (corresponding to amino acids 369412) was amplified with primers 2 and 4 (5'-CATGCAAAGAGTTCTTGATCTTGAATTTTTCCC-3'). The two fragments were then combined, diluted and amplified using primers 1 and 2 to reconstruct the complete cDNA. The mutagenesis of tor-2 was confirmed by DNA sequencing. | ||
| Genetic_information | Integrated | ||
| Associated_with | Strain | WBStrain00035176 | |
| Reference | WBPaper00005722 | ||
| Species | Caenorhabditis elegans |
