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WormBase Tree Display for Strain: WBStrain00055444

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Name Class

WBStrain00055444GenotypeT20D3.6(ve796[LoxP + myo-2p::GFP::unc-54 3' UTR + rps-27p::neoR::unc-54 3' UTR + LoxP]) IV.
Public_nameRG3296
ContainsGeneWBGene00003514
WBGene00004496
WBGene00006789
PropertiesOutcrossedx0
MutagenCRISPR_Cas9
CGC_received23 Feb 2022 00:00:00
LocationCGC
RemarkHomozygous viable. Deletion of 526 bp with Calarco/Colaiacovo selection cassette conferring myo-2 GFP and G418 resistance inserted at break in parental strain N2. Left flanking Sequence: tagaacattttatgaatcaaaagTTAAGCA ; Right flanking sequence: CGGAAGTCATGTGGCTCGGCGTTCATtttt. sgRNA #1: GAATTATTCTGAGGAACCGC; sgRNA #2: TTATATGCGCTTTGTGATCA. Please reference Au et al., G3 9(1): 135-144 2019 in any work resulting from use of this mutation.Inferred_automaticallyFrom CGC strain data
Made_by: RG KO GroupCGC_data_submission
SpeciesCaenorhabditis elegans