WormBase Tree Display for Strain: WBStrain00052050
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| WBStrain00052050 | Status | Live | ||
|---|---|---|---|---|
| Genotype | rab-5(udn64)/tmC18 [dpy-5(tmIs1236)] I; udnSi38 II. | |||
| Public_name | UDN100126 | |||
| Contains | Gene | WBGene00001067 | ||
| WBGene00004268 | ||||
| Rearrangement | tmC18 | |||
| Transgene | WBTransgene00024753 | |||
| Properties | Outcrossed | x2 | ||
| Mutagen | CRISPR_Cas9 | |||
| CGC_received | 30 Mar 2022 00:00:00 | |||
| Location | CGC | |||
| Remark | udnSi38 [rab5p::rab-5] II. Maintain at 20 degrees. rab-5 [D135N] variant edit #2 with a single copy of wild-type rab-5 integrated into chromosome II at ttTi5605 site (II: 0.77). Homozygous lethal rab-5 [D135N] mutation balanced by tmC18. Balancer marked with myo-2p::mCherry. Heterozygotes are WT with pharyngeal mCherry fluorescence, and segregate mCherry + heterozygotes, non-mCherry rab-5 [D135N] homozygotes (L1 lethal), and Dpy mCherry+ tmC18 homozygotes. Pick fertile wild-type mCherry+ to maintain. [D135N]/ [D135N]; udnSi38/udnSi38 double homozygotes are lethal. Reference: Huang et al. 2022. PMID: 35121658 | Inferred_automatically | From CGC strain data | |
| Made_by: UDN Screening Center | CGC_data_submission | |||
| Species | Caenorhabditis elegans |
