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WormBase Tree Display for Strain: WBStrain00051447

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Name Class

WBStrain00051447StatusLive
GenotypeY65B4A.8(gk5849[loxP + myo-2p::GFP::unc-54 3' UTR + rps-27p::neoR::unc-54 3' UTR + loxP])/+ I.
Public_nameVC4781
ContainsGeneWBGene00003514
WBGene00004496
WBGene00006789
VariationWBVar02156678
PropertiesOutcrossedx0
MutagenCRISPR_Cas9
CGC_received25 Jan 2021 00:00:00
LocationCGC
RemarkApparent homozygous lethal or sterile deletion as unbalanced heterozygote. Deletion of 3386 bp with Calarco/Colaiacovo selection cassette conferring myo-2 GFP and G418 resistance inserted at break. Pick viable fertile GFP+ animals to maintain. Left flanking sequence: GACGGTCCACGTTTCGGCGAGCGTTTTGTG. Right flanking sequence: CGGCTGCGCGTCTCTATTTCACACACTGTC. Please reference Au et al., G3 9(1): 135-144 2019 in any work resulting from use of this mutation.Inferred_automaticallyFrom CGC strain data
Made_by: Vancouver KO GroupCGC_data_submission
SpeciesCaenorhabditis elegans