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WormBase Tree Display for Strain: WBStrain00051218

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Name Class

WBStrain00051218StatusLive
Genotypeeif-2Bepsilon(gk5600[loxP + myo-2p::GFP::unc-54 3' UTR + rps-27p::neoR::unc-54 3' UTR + loxP])/+ II.
Public_nameVC4529
ContainsGeneWBGene00003514
WBGene00004496
WBGene00006789
VariationWBVar02156446
PropertiesOutcrossedx0
MutagenCRISPR_Cas9
CGC_received31 Aug 2020 00:00:00
LocationCGC
RemarkMade_by: Vancouver KO GroupCGC_data_submission
[NOTE: Please see RG5013 for balanced version of this strain.] Apparent homozygous lethal or sterile deletion as unbalanced heterozygote. Deletion of 2890 bp with Calarco/Colaiacovo selection cassette conferring myo-2 GFP and G418 resistance inserted at break. Pick viable fertile GFP+ animals to maintain. Left flanking sequence: AAAATTTTGCAGATGCAATGACGCCCTACC. Right flanking sequence: CTTGTTATGACTGAAAGTTTTCAACCACTT. Please reference Au et al., G3 9(1): 135-144 2019 in any work resulting from use of this mutation.Inferred_automaticallyFrom CGC strain data
SpeciesCaenorhabditis elegans