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WormBase Tree Display for Strain: WBStrain00049473

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Name Class

WBStrain00049473EvidenceCurator_confirmedWBPerson1983
StatusLive
GenotypeY40B1A.1(ve736[LoxP + myo-2p::GFP::unc-54 3' UTR + rps-27p::neoR::unc-54 3' UTR + LoxP]) I.
Public_nameRG3236
ContainsGeneWBGene00003514
WBGene00004496
WBGene00006789
VariationWBVar02154291
PropertiesOutcrossedx0
MutagenCRISPR_Cas9
CGC_received27 Sep 2021 00:00:00
LocationRG
CGC
RemarkHomozygous viable. Deletion of 2839 bp with Calarco/Colaiacovo selection cassette conferring myo-2 GFP and G418 resistance inserted at break in parental strain N2. Left flanking Sequence: gagcctcgaaaactccccaaaaactatacc ; Right flanking sequence: tttcgtgtatttttccgtttgtgtgcaaaa. sgRNA #3: cgtcttgtagatcagctcgg; sgRNA #4: agagatcatcactggaatgg. Please reference Au et al., G3 9(1): 135-144 2019 in any work resulting from use of this mutation.Inferred_automaticallyFrom CGC strain data
Made_by: RG KO GroupCGC_data_submission
SpeciesCaenorhabditis elegans