WormBase Tree Display for Strain: WBStrain00049395
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WBStrain00049395 | Evidence | Curator_confirmed | WBPerson1983 | |
---|---|---|---|---|
Status | Live | |||
Genotype | +/nT1 [umnIs49] IV; F53F1.2(ve656[LoxP + myo-2p::GFP::unc-54 3' UTR + rps-27p::neoR::unc-54 3' UTR + LoxP])/nT1 V. | |||
Public_name | RG3156 | |||
Contains | Gene | WBGene00003514 | ||
WBGene00004496 | ||||
WBGene00006789 | ||||
Variation | WBVar02154214 | |||
Rearrangement | nT1 | |||
Transgene | WBTransgene00033215 | |||
Properties | Outcrossed | x0 | ||
Mutagen | CRISPR_Cas9 | |||
CGC_received | 17 Nov 2020 00:00:00 | |||
Location | CGC | |||
Remark | umnIs49 [myo-2p::mKate2 + NeoR, V: 1005689 (intergenic)] IV. Homozygous Mel. Deletion of 1448 bp with Calarco/Colaiacovo selection cassette conferring myo-2 GFP and G418 resistance inserted at break in parental strain N2. Heterozygotes are wild-type GFP+ mKate2+, and segregate wild-type GFP+ mKate2+, GFP+ non-mKate adults that lay dead eggs (ve656 homozygotes), Vul non-GFP mKate2+ (nT1 homozygotes) and dead eggs (aneuploids). Maintain by picking wild-type GFP+ mKate2+. Left flanking Sequence: aataacttttgctgtccgctcattcctgat ; Right flanking sequence: tcgcaatattccgctataatatctttagtt. sgRNA #1: gaatcgaggtcagtcgcatc; sgRNA #2: atagcggaatattgcgagag. Please reference Au et al., G3 9(1): 135-144 2019 in any work resulting from use of this mutation. | |||
umnIs49 [myo-2p::mKate2 + NeoR, V: 1005689 (intergenic)] IV. Homozygous Mel. Deletion of 1448 bp with Calarco/Colaiacovo selection cassette conferring myo-2 GFP and G418 resistance inserted at break in parental strain N2. Heterozygotes are wild-type GFP+ mKate2+, and segregate wild-type GFP+ mKate2+, GFP+ non-mKate adults that lay dead eggs (ve656 homozygotes), Vul non-GFP mKate2+ (nT1 homozygotes) and dead eggs (aneuploids). | Inferred_automatically | From CGC strain data | ||
Made_by: RG KO Group | CGC_data_submission | |||
Species | Caenorhabditis elegans |