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WormBase Tree Display for Strain: WBStrain00030553

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Name Class

WBStrain00030553StatusLive
GenotypeCaenorhabditis elegans wild isolate.
Public_namePD1074
PropertiesOutcrossedx0
CGC_received01 Jan 2019 00:00:00
PhenotypeWBPhenotype:0000481Paper_evidenceWBPaper00065019
Curator_confirmedWBPerson712
RemarkPD1074 animals were indifferent to solvent and extracts from the Velvety tree ants but avoided Argentine ant extracts (PD1074: solvent vs Argentine ant extract, t(40) = 4.223, p = 0.003).Paper_evidenceWBPaper00065019
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00008002Paper_evidenceWBPaper00065019
Curator_confirmedWBPerson712
WBPhenotype:0002109Paper_evidenceWBPaper00060848
Curator_confirmedWBPerson712
RemarkOur data show that at 1-HP experimental concentrations of 222 uM, 111 uM, and 55.6 uM, mortality rates are approximately 60%, 40%, and 20%, respectively. The difference of mortality rates between the control and lower concentrations were statistically insignificant (Figure 1E). We performed a Tukey's honestly significant difference (HSD) test using JMP statistical software that showed that the death at 222 uM was statistically significant compared to all other toxin concentrations in our range (Figure 1E). We also found that the death rate at 111 uM was statistically significant when compared to the death rates at the other toxin concentrations and controls except for 55.6 uM (Figure1E). The data show that 1-HP is toxic to PD1074 at similar concentration ranges on plates as it was to N2 in liquid (Stupp et al., 2013).Paper_evidenceWBPaper00060848
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00001441Paper_evidenceWBPaper00060848
Curator_confirmedWBPerson712
Phenotype_not_observedWBPhenotype:0000072Paper_evidenceWBPaper00060382
Curator_confirmedWBPerson712
RemarkWe report that lifelong exposure to 0.025mM or 1mM Auxin does not significantly affect any of the objectively quantified phenotypic features in either N2 or PD1074 wild-type strains (Figure 1D-G). Body length and width were no different among animals exposed to 0 to 1mM auxin exposure.Paper_evidenceWBPaper00060382
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00007857Paper_evidenceWBPaper00060382
Curator_confirmedWBPerson712
WBPhenotype:0000481Paper_evidenceWBPaper00065019
Curator_confirmedWBPerson712
RemarkExtracts of the Velvety tree ant showed a different pattern of behavioral response, in which tax-4 mutants were repelled by the extracts (tax-4: solvent vs Velvety tree ant extract, t(40) = 5.645, p < 0.001), but osm-9 mutants (osm-9: solvent vs Velvety tree ant extract, t(40) = 0.018, p = 0.985) and the wild type (PD1074: solvent vs Velvety tree ant extract, t(40) = 1.096, p = 0.373) were not.Paper_evidenceWBPaper00065019
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00008001Paper_evidenceWBPaper00065019
Curator_confirmedWBPerson712
WBPhenotype:0000643Paper_evidenceWBPaper00060382
Curator_confirmedWBPerson712
RemarkWe report that lifelong exposure to 0.025mM or 1mM Auxin does not significantly affect any of the objectively quantified phenotypic features in either N2 or PD1074 wild-type strains (Figure 1D-G). Locomotion speed and bias were no different among animals exposed to 0 to 1mM auxin exposure.Paper_evidenceWBPaper00060382
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00007857Paper_evidenceWBPaper00060382
Curator_confirmedWBPerson712
WBPhenotype:0001703Paper_evidenceWBPaper00060382
Curator_confirmedWBPerson712
RemarkWe report that lifelong exposure to 0.025mM or 1mM Auxin does not significantly affect any of the objectively quantified phenotypic features in either N2 or PD1074 wild-type strains (Figure 1D-G). Degree of kink in body posture were no different among animals exposed to 0 to 1mM auxin exposure.Paper_evidenceWBPaper00060382
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00007857Paper_evidenceWBPaper00060382
Curator_confirmedWBPerson712
WBPhenotype:0002320Paper_evidenceWBPaper00060382
Curator_confirmedWBPerson712
RemarkWe report that lifelong exposure to 0.025mM or 1mM Auxin does not significantly affect any of the objectively quantified phenotypic features in either N2 or PD1074 wild-type strains (Figure 1D-G). Reversal probability, speed and duration were no different from no to 1mM auxin exposure.Paper_evidenceWBPaper00060382
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00007857Paper_evidenceWBPaper00060382
Curator_confirmedWBPerson712
LocationCGC
PD
RemarkA defined and recently cloned population of animals derived from the original Bristol variant of C. elegans originally obtained by Brenner from E. Dougherty with no known history of mutagenesis. Brenners original population, called N2, was used as the basis for the vast majority of laboratory strains in use currently. No early frozen stock of the unmutagenized N2 population currently exists, but later stocks were available from several laboratories. PD1074 is a clonal population founded by picking a single worm of one such stock, VC3510. VC3510 in turn derives from a subpopulation of N2 described in the literature as VC2010. PD1074 is intended to be used as a wild type reference strain with the closely matched genome assembly of Yoshimura et al (ref) available on Wormbase as VC2010-1.0. (ENA study accession PRJEB28388; assembly accession GCA_900538205). We note that PD1074 is expected to be largely similar to most lab N2 strains, but that as a clonal isolate derived from N2, there will be some loci that will vary compared to any other particular N2 isolate. One such example is a partial deletion of the alh-2 locus in PD1074. Additional loci that were found to vary between the prior N2 reference genome (WormBase release WS264) and the VC2010-1.0 assembly are detailed in supplemental table 8 in Yoshimura et al (ref).Inferred_automaticallyFrom CGC strain data
Made_by: Karen Artiles & Mark EdgleyCGC_data_submission
A defined and recently cloned population of animals derived from the original Bristol variant of C. elegans originally obtained by Brenner from E. Dougherty with no known history of mutagenesis. Brenners original population, called N2, was used as the basis for the vast majority of laboratory strains in use currently. No early frozen stock of the unmutagenized N2 population currently exists, but later stocks were available from several laboratories. PD1074 is a clonal population founded by picking a single worm of one such stock, VC3510. VC3510 in turn derives from a subpopulation of N2 described in the literature as VC2010. PD1074 is intended to be used as a wild type reference strain with the closely matched genome assembly of Yoshimura, et al. (Genome Res. 2019 Jun;29(6):1009-1022) available on Wormbase as VC2010-1.0. (ENA study accession PRJEB28388; assembly accession GCA_900538205). We note that PD1074 is expected to be largely similar to most lab N2 strains, but that as a clonal isolate derived from N2, there will be some loci that will vary compared to any other particular N2 isolate. One such example is a partial deletion of the alh-2 locus in PD1074. Additional loci that were found to vary between the prior N2 reference genome (WormBase release WS264) and the VC2010-1.0 assembly are detailed in supplemental table 8 in Yoshimura, et al, (2019).Inferred_automaticallyFrom CGC strain data
Added Wild_Isolate tag as genotype info suggesting that they were sampled from the wild.Curator_confirmedWBPerson1983
WBStrain provided so WBPaper00061573 paper added based on AFP_Strain data.Curator_confirmedWBPerson1983
WBStrain provided so WBPaper00061243 paper added based on AFP_Strain data.Curator_confirmedWBPerson1983
no longer available from the CGC.
ReferenceWBPaper00060382
WBPaper00060848
WBPaper00061573
WBPaper00061243
WBPaper00065352
WBPaper00065279
WBPaper00065019
SpeciesCaenorhabditis elegans
Wild_isolate