WormBase Tree Display for RNAi: WBRNAi00102856
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| WBRNAi00102856 | Homol | Homol_homol | F59F4:RNAi | ||
|---|---|---|---|---|---|
| Sequence_info | PCR_product | sjj_F59F4.1 | |||
| Experiment | Laboratory | KQ | |||
| Date | 06 Dec 2013 00:00:00 | ||||
| Treatment | Synchronized L1 animals were transferred to plates containing RNAi/bacteria. They were fed on RNAi plates throughout their growth and treatment. The identity of each RNAi clone was blinded during experiments. Knockdowns that resulted in a suppression of the dopamine induced fat phenotype of more than 80% of the control animal's reduction were considered for further analyses. For the metabolic screen, data were acquired with Acumen (TTP LABTECH, England) a microplate fluorescence citometer, and double checked in a visual-based assay. For missing values or values with SD bigger than 20% from the automatic assay, only the visual-based assay data were considered. Data from both assays had the same trend with the former being more sensitive for variations in intensity. | ||||
| Delivered_by | Bacterial_feeding | ||||
| Inhibits | Predicted_gene | F59F4.1 | Inferred_automatically | RNAi_primary | |
| Gene | WBGene00010336 | Inferred_automatically | RNAi_primary | ||
| Transcript | F59F4.1.1 | Inferred_automatically | RNAi_primary | ||
| Species | Caenorhabditis elegans | ||||
| Reference | WBPaper00044757 | ||||
| Phenotype_not_observed | WBPhenotype:0001184 | Remark | F59F4.1 RNAi did not lead to fat accumulation | ||
| Remark | (Data not shown) F59F4.1 RNAi | ||||
| Method | RNAi |
