WormBase Tree Display for RNAi: WBRNAi00102620

WBRNAi00102620 Homol: Homol_homol: F01G10:RNAi
  Sequence_info: PCR_product: sjj_F01G10.3
  Experiment: Laboratory: KQ
    Date: 29 Apr 2008 00:00:00
    Treatment: HT115 bacteria containing each RNAi vector were tested as described previously (Ashrafi et al., 2003) with the following modification: after overnight growth, bacteria were pelleted and resuspended to 5x concentration, 0.1 ml of which was used for seeding each well of a 12-well plate containing 4 ml NGM agar, 6 mM IPTG, and 25 mg/ml carbenicillin. Next, Nile red and 5-HT or HCl vehicle were added on top of each well, and the plates were allowed to equilibrate overnight. Twenty to thirty synchronized wild-type or mutant L1 animals were placed per well and incubated at 20 degrees Celsius to adulthood. Nile red assessment was performed on gravid adult animals. For each RNAi experiment, HT115 (vector alone) and OP50 control wells were also included. All 5-HT suppression phenotypes were confirmed by multiple (5-10) additional rounds of testing on the selected clones, whose identities were confirmed by DNA sequencing. At the time of scoring Nile red phenotypes, the identities of the target RNAi clones were unknown.
    Delivered_by: Bacterial_feeding
  Inhibits: Predicted_gene: F01G10.3a Inferred_automatically: RNAi_primary
      F01G10.3b Inferred_automatically: RNAi_primary
    Gene: WBGene00001158 Inferred_automatically: RNAi_primary
    Transcript: F01G10.3b.1 Inferred_automatically: RNAi_primary
      F01G10.3a.1 Inferred_automatically: RNAi_primary
  Species: Caenorhabditis elegans
  Reference: WBPaper00031915
  Phenotype_not_observed: WBPhenotype:0001182 Remark: RNAi did not affect the fat-reducing effects of serotonin (5-HT)
      Affected_by: Molecule: WBMol:00004929
  Remark: (Data not shown) F01G10.3 RNAi. Exact sequence used for RNAi not stated by authors, Ahringer laboratory clone used for curation.
  Method: RNAi