WormBase Tree Display for RNAi: WBRNAi00102337
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| WBRNAi00102337 | Homol | Homol_homol | D2024:RNAi | ||
|---|---|---|---|---|---|
| Sequence_info | PCR_product | mv_D2024.7 | |||
| Experiment | Laboratory | RD | |||
| Date | 07 Jul 2009 00:00:00 | ||||
| Genotype | Pgstk-1::GFP::GSTK-1 | ||||
| Treatment | RNAi strain cultures were grown for 6 hours in LB medium containing 100 mg/mL ampicillin, and then spread onto NGM agar containing isopropyl thio-beta-D-galactoside (1 mM) and carbenicillin (25 micrograms per millilter). For double- RNAi treatment, equal concentrations of both strains were mixed before seeding. The next day, animals at the fourth larval stage were placed onto RNAi plates, grown for 2 days and then harvested by rinsing with M9 buffer (0.1 M NaCl, 0.05 M potassium phosphate, pH 6.0). Adults were allowed to settle, and eggs were recovered from hermaphrodites by alkaline hypochlorite lysis (5 min at room temperature in 0.5 M NaOH, 5% hypochlorite). The eggs were rinsed with M9 buffer and the resulting L1 larvae were transferred the next day to fresh agar plates containing the different dsRNA conditions. | ||||
| Delivered_by | Bacterial_feeding | ||||
| Inhibits | Predicted_gene | D2024.7 | Inferred_automatically | RNAi_primary | |
| Gene | WBGene00017054 | Inferred_automatically | RNAi_primary | ||
| Transcript | D2024.7.1 | Inferred_automatically | RNAi_primary | ||
| Species | Caenorhabditis elegans | ||||
| Reference | WBPaper00035091 | ||||
| Phenotype_not_observed | WBPhenotype:0000306 | Remark | gstk-2 RNAi does not affect expression of the GFP::GSTK-1 transgene | ||
| Remark | (Figure S1B) gstk-2 RNAi | ||||
| Method | RNAi |
