WormBase Tree Display for RNAi: WBRNAi00101481
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| WBRNAi00101481 | Homol | Homol_homol | C16B8:RNAi | ||
|---|---|---|---|---|---|
| Sequence_info | DNA_text | ATGATTCTTCGCTACCTGATTTTTTTCGCGCAGTTATGGGCACTCTGCCTGGCCAATGTGAATATGTTCATCAGCAAAGAGGAAATGAATCGTACTTTTGGTGTCAAAGCTGAGCTGAATTACATTGAAATGGGGAATGTTAGCTCGTACTCTACAAAGTTTCACTACAGAGTTATGGCAAACATCGACTACCTCTCGTTCACATGGAATGCTGTTGGAATTGTACACTATGAAGTTTACGTCGAATCTGATGACTCTTCTGTGCTTCCTATTGTTCGAATTCCATTGAAAGGAACGGTGCCAGAATCTTTGCAGGACTTCACCGTTGAATACAGATGTGCCGGACACCGATCCGGACAATTTGCTGTCAGTCTATATTTCACATTCAAATATGGTAATAAGGAGCCGTTGAAAGTGAAATTGCGACAGGAGAAGATCTGCGCTTCAAGGGACGGACGTCGAGGTCTGAACGGAGGCTACGAGGGTCATGAAGTCGACGACACTGACTCAATAGACAAGGCATTTTTTGTTATCATTTGCATTGCTGCGGCATTCCTACTTATTGTGGCAGCAACGTTGATCTGTTATTTCAAGCGCTCTAAAAAAGAAGACATGATTCCGACTCGACTTCCAACGTCTTTTCGGAATTCTTTGAAATCTACAAAAAGCGCGCAGCCTTTTCTTCTGAGCACACCGCGAGATGGACCTCCGACTCTTTCCGCTATTTCAAGCGCTCCTTGTTCTTCGTCGTCTGCGTCGGGAAATTCGATAATCCCGAGCAAGCCAAGAAACATTGACGTGAGACGTGCATTGTTACAACTCTATCAAGATCGAGATGCTTTTCAATCTCTACCTCTAGATATGGAGGGAACATTTGGAGAAGTGAGATATGCAATTTGGCGTCAAGTAGATGACGTACTGAACGGAGATGTTGACGACGAAGAAGACACATTCTGTAACCAGGAAGCTGTTTACACCAAAACGTTGAAAAATAATGCCTCACCAATTCAGCTGGATCGGTTTTTGTCCGACGCCCTTCTATTTTACAACATCACACCTCACCAAAACTTGTCTCAAGTGGCATGTGTGGCTTCCTTCGGAAGATTCGACCGCCCGGAAACTGTCACAGATTTTCCACTTGTTTGTTACAGACACCAAGGCTTTGGAAACCTGAAGAAGTTCCTCACCATCTGCCGACATGGTGATAAAACTAAAGGAGCTCAAACTCTCCGAACTCATCAACTCGTCTCTCTGGCCACACAAGTATCTTCTGCAGTAGCTCATATACACAAATATAGAATAGTGCATAACGACATTGCCGCTAGAAACTGCTTGATCGCAGAAGTGAATGGGCGACTCCAAGTGCAATTATGCGACTCGGCGCTGTCCCGCGATCTGTTCCCAGCTGATTATCACTGCTTGGGTGACAATGAGAACAGACCATTGAAATGGATGTCTCCAGAAGCTATTGCAAATGAGCTGTACTCATCGGCCGCTGATGTTTGGTCACTGGGAGTTCTACTGTGGGAGCTCATGTCGCTAGGAGGATCTCCACACGCTGAAATAGACCCTGAGGAAGTGTACACAATGATTCTCAAAGGAAAGCGTCTGCAACAGCCGAACAATTGTCCGGATCAATTATACGAAGTCATGCTGTGCTGTTGGAGGGTACTCAGCGAAGATCGTCCTAGCAGTGAGCAGGTAGTTCATGGACTTCGAGACTTTAACATTCAACTCAGTCAATACATCTAA | |||
| Experiment | Laboratory | CT | |||
| Date | 01 Nov 2010 00:00:00 | ||||
| Genotype | wIs78 [SCMp::GFP marker transgene for seam cells] | ||||
| Treatment | RNAi was induced by feeding, starting with L1 stage starved and synchronized animals. RNAi constructs were expressed from E. coli HT115(DE3) bacteria, which were grown on NGM media containing 1 mM IPTG to induce expression from the convergent T7 polymerase promoters on the pL4440 vector. All strains feeding on RNAi bacteria were cultured at 20 degrees Celsius unless otherwise noted. Simultaneous RNAi was carried out by growing the two different RNAi bacterial strains to the same optical density and mixing equal volumes of the cultures before plating onto NGM plates. | ||||
| Temperature | 20 | ||||
| Delivered_by | Bacterial_feeding | ||||
| Inhibits | Predicted_gene | C16B8.1 | Inferred_automatically | RNAi_primary | |
| Gene | WBGene00003007 | Inferred_automatically | RNAi_primary | ||
| Transcript | C16B8.1.1 | Inferred_automatically | RNAi_primary | ||
| Species | Caenorhabditis elegans | ||||
| Reference | WBPaper00037838 | ||||
| Phenotype_not_observed | WBPhenotype:0001887 | ||||
| WBPhenotype:0001923 | |||||
| Remark | (Table 1) lin-18/empty-vector double RNAi. Exact sequence used for RNAi not stated by authors, spliced coding region sequence of gene used for curation. | ||||
| Method | RNAi |
