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WormBase Tree Display for RNAi: WBRNAi00097923

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Name Class

WBRNAi00097923HomolHomol_homolF52B5:RNAi
Sequence_infoDNA_textgttcgaattgttgcctatccacgacgtgactggaagaatttctgtgagcgagaagacgcaaaacaaaaggatttcagatttcccgagttacctgcctacaagaaggcgagcctagaatcgataaatatcaaacaagaggtcaatctagagaacatgttcaacgtgaccaatactactgcacaggtttgtctagaatatgatttaaaagaatttggaaaatagtaaataacttcaatttccaaaatcatgccttgtagaaatatcaattttctgccagaagaatataccatatttatcatgattcttttcaattttctatagaaaaacgttttcttcttgtttccgtcgaatgaacaaaaactgaattgaaattcgatatcttatctcccaatgacttcctttatgcgtaatgtttgctcgaccacaaagctttgaaatgttttatttcgatctaattttttaaatatatatgctctaattcccaagacattcaattattttctcaacgcaatatgaaataagattttaaatttacagatggaaccatcaacttcatattcatctccatcaaacagtaataatcggaagagatttttgaatgagtgtgattctccaaataatgattatacaatgatgcacagaactccaccagtaacaggttatgcaagtcgtcttcatggatgcgttcctccgattgaaactgaacacgaaaactgtcaatctccgtcgatgaagagaagtcgctgtaccaattattcgtttagaacgctcactgtaagtatttttagctcactcttaaagcaatattataattaattttgattcacaataaatttcagctgtcgactgctgagtatacaaaagtcgtcgaatttctggcacgcgaagcaaaagttcccagatacacttgggttccgacgcaagtagtctcccatatattgccaactgaaggacttgaaaggtatttataaagatttataaagattgccgtaatttcttttttttgaagattcctcaccgctataaaagcagggcacgattcagtgttgttcaatgcaaacggaatttatacaatgggggatatgattagagaattcgagaaacataatgacatcttcgaaagaattggtatcgattcttcgaaattgtcgaaatactacgaagcgtttctcagcttttaccgcatccaggaagcgatga
ExperimentLaboratoryTG
Date27 Sep 2001 00:00:00
TreatmentApproximately three P0 worms were added to the RNAi plate and incubated at 15 degrees Celsius for 72 hours. Three single F1 worms were each transferred into a new, freshly seeded plate and were allowed to lay eggs for approximately 20 hr. F1 worms were aspirated, and F2 worms were allowed to grow up to the L4 stage, irradiated with an X-ray source, and mitotic germlines analyzed for radiation-induced cell cycle arrest.
Delivered_byBacterial_feeding
InhibitsPredicted_geneF52B5.5aInferred_automaticallyRNAi_primary
F52B5.5bInferred_automaticallyRNAi_primary
GeneWBGene00000467Inferred_automaticallyRNAi_primary
TranscriptF52B5.5b.1Inferred_automaticallyRNAi_primary
F52B5.5b.4Inferred_automaticallyRNAi_primary
F52B5.5b.2Inferred_automaticallyRNAi_primary
F52B5.5a.1Inferred_automaticallyRNAi_primary
F52B5.5b.3Inferred_automaticallyRNAi_primary
SpeciesCaenorhabditis elegans
ReferenceWBPaper00004952
Phenotype_not_observedWBPhenotype:0002179Remarkcep-1 RNAi did not affect mitotic germ cell arrest in response to X-ray radiation
Remark(Figure S1a) cep-1 RNAi
MethodRNAi