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WormBase Tree Display for RNAi: WBRNAi00097917

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Name Class

WBRNAi00097917HomolHomol_homolC35D10:RNAi
Sequence_infoPCR_productsjj_C35D10.9
ExperimentLaboratoryTG
Date27 Sep 2001 00:00:00
TreatmentApproximately three P0 worms were added to the RNAi plate and incubated at 15 degrees Celsius for 72 hours. Three single F1 worms were each transferred into a new, freshly seeded plate and were allowed to lay eggs for approximately 20 hr. F1 worms were aspirated, and F2 worms were allowed to grow up to the L4 stage, irradiated with an X-ray source, and analyzed for radiation-induced cell cycle arrest and apoptosis. Germlines were scored for corpses 36 hours after 60 Gray irradiation.
Delivered_byBacterial_feeding
InhibitsPredicted_geneC35D10.9bInferred_automaticallyRNAi_primary
C35D10.9aInferred_automaticallyRNAi_primary
GeneWBGene00000418Inferred_automaticallyRNAi_primary
TranscriptC35D10.9b.1Inferred_automaticallyRNAi_primary
C35D10.9a.1Inferred_automaticallyRNAi_primary
SpeciesCaenorhabditis elegans
ReferenceWBPaper00004952
PhenotypeWBPhenotype:0002541Remarkced-4 RNAi resulted in worms with dramatically reduced numbers of apoptotic germ cell corpses in response to 60 Gray radiation
Remark(Data not shown) ced-4 RNAi. Authors state RNAi clones obtained from the Ahringer and Vidal laboratories, Ahringer clone used for curation.
MethodRNAi