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WormBase Tree Display for RNAi: WBRNAi00097254

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Name Class

WBRNAi00097254HomolHomol_homolY40B1B:RNAi
CHROMOSOME_I:RNAi
Sequence_infoPCR_productsjj_Y40B1B.7
ExperimentLaboratoryXF
Date08 May 2006 00:00:00
TreatmentSynchronized wild-type L1 worms were grown in liquid culture with bacteria expressing dsRNA for 2 days. L4 animals were exposed to 0.3 mM cis-diamminedichloroplatinum (cisplatin) (Platosin dissolved in 0.9% NaCl, Pharmachemie BV) in M9 for 3 hr. Control animals were mock-treated with 0.9% NaCl in M9. After treatment, the animals were transferred to plates with the original RNAi food. Twenty-four hours later four sets of three worms were transferred to a second plate to allow egg laying. The parents were removed after 18 hr. Dead eggs and offspring were counted 24 hr thereafter.
Delivered_byBacterial_feeding
InhibitsPredicted_geneY40B1B.7Inferred_automaticallyRNAi_primary
GeneWBGene00012289Inferred_automaticallyRNAi_primary
WBGene00012739Inferred_automaticallyRNAi_primary
TranscriptY40B1B.7.1Inferred_automaticallyRNAi_primary
W05H12.1.1Inferred_automaticallyRNAi_primary
SpeciesCaenorhabditis elegans
ReferenceWBPaper00027740
Phenotype_not_observedWBPhenotype:0001918RemarkRNAi did not result in hypersensitivity to the drug cisplatin
Affected_byMoleculeWBMol:00002867
Remark(Table 1) Y40B1B.7 RNAi
MethodRNAi