WormBase Tree Display for RNAi: WBRNAi00094343
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| WBRNAi00094343 | Homol | Homol_homol | Y49F6B:RNAi | ||
|---|---|---|---|---|---|
| Sequence_info | PCR_product | sjj_Y49F6B.q | |||
| Experiment | Laboratory | HZ | |||
| Date | 18 Oct 2011 00:00:00 | ||||
| Genotype | bpIs70 [gfp::bro-1(PTC)] | ||||
| Treatment | Five to seven L4 animals carrying a gfp::bro-1(PTC) transgene were plated onto RNAi feeding plates and were grown at 21 degrees Celsius for 3 days. The F1 L3 or L4 larvae were examined for reporter expression. Expression of gfp::bro-1(PTC) was not detected in late larvae fed on control RNAi clone (n > 100). The ratio of animals expressing gfp::bro-1(PTC) was derived from three separate experiments (at least 30 animals were examined for each RNAi clone). The RNAi clones that caused more than 25% of animals expressing gfp were selected for further analysis. | ||||
| Temperature | 21 | ||||
| Delivered_by | Bacterial_feeding | ||||
| Inhibits | Predicted_gene | Y49F6B.2 | Inferred_automatically | RNAi_primary | |
| Gene | WBGene00021715 | Inferred_automatically | RNAi_primary | ||
| Transcript | Y49F6B.2.1 | Inferred_automatically | RNAi_primary | ||
| Species | Caenorhabditis elegans | ||||
| Reference | WBPaper00040559 | ||||
| Phenotype | WBPhenotype:0000508 | Remark | RNAi resulted in increased larval expression of a gfp::bro-1(PTC) transgene carrying a premature termination codon, indicative of defective mRNA surveillance machinery | ||
| WBPhenotype:0001236 | Remark | RNAi resulted in increased larval expression of a gfp::bro-1(PTC) transgene carrying a premature termination codon, indicative of defective mRNA surveillance machinery | |||
| WBPhenotype:0001538 | Remark | RNAi resulted in increased larval expression of a gfp::bro-1(PTC) transgene carrying a premature termination codon, indicative of defective mRNA surveillance machinery | |||
| Remark | (Table S1) Y49F6B.2 RNAi | ||||
| Method | RNAi |
