WormBase Tree Display for RNAi: WBRNAi00092627
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| WBRNAi00092627 | Homol | Homol_homol | ZK1320:RNAi | ||
|---|---|---|---|---|---|
| Sequence_info | PCR_product | sjj_ZK1320.4 | |||
| Experiment | Laboratory | MQ | |||
| Date | 09 Jan 2012 00:00:00 | ||||
| Genotype | clk-1(qm30) | ||||
| Treatment | 5-10 clk-1(qm30) hermaphrodite L4 larvae were picked to RNAi plates. For the following 3 days, worms were transferred to new RNAi plates to rid of contaminating OP50 bacteria. Progeny worms were grown to the L4 stage and were then picked to new RNAi plates for scoring. 18 hours later, they were transferred to 25 degrees Celsius. After two hours of acclimation, their defecation phenotype was scored. For each RNAi clone, five worms were scored for one defecation cycle. Clones that had a significant effect on defecation rate were re-screened 2-3 times. | ||||
| Temperature | 25 | ||||
| Delivered_by | Bacterial_feeding | ||||
| Inhibits | Predicted_gene | ZK1320.4 | Inferred_automatically | RNAi_primary | |
| Gene | WBGene00014254 | Inferred_automatically | RNAi_primary | ||
| Transcript | ZK1320.4.1 | Inferred_automatically | RNAi_primary | ||
| Species | Caenorhabditis elegans | ||||
| Reference | WBPaper00040902 | ||||
| Phenotype_not_observed | WBPhenotype:0000246 | Remark | RNAi did not significantly affect the defecation rate of clk-1(qm30) mutants | ||
| Remark | (Table 1) cyp-13A10 RNAi. Exact sequence used for RNAi not stated by authors, Ahringer laboratory clone used for curation. | ||||
| Method | RNAi |
