WormBase Tree Display for RNAi: WBRNAi00092397
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| WBRNAi00092397 | Homol | Homol_homol | T21G5:RNAi | ||
|---|---|---|---|---|---|
| Sequence_info | PCR_product | sjj_T21G5.5 | |||
| Experiment | Laboratory | KH | |||
| Date | 10 Aug 2012 00:00:00 | ||||
| Genotype | asd-2(yb1540); unc-60(su158) | ||||
| Treatment | For RNAi experiment by feeding, L4 hermaphrodites were transferred to agar plates seeded with bacteria expressing dsRNAs of target genes and their progeny were scored for colour and behavioural phenotypes or used for staining. | ||||
| Delivered_by | Bacterial_feeding | ||||
| Inhibits | Predicted_gene | T21G5.5d | Inferred_automatically | RNAi_primary | |
| T21G5.5b | Inferred_automatically | RNAi_primary | |||
| T21G5.5a | Inferred_automatically | RNAi_primary | |||
| T21G5.5c | Inferred_automatically | RNAi_primary | |||
| Gene | WBGene00006423 | Inferred_automatically | RNAi_primary | ||
| Transcript | T21G5.5d.2 | Inferred_automatically | RNAi_primary | ||
| T21G5.5b.1 | Inferred_automatically | RNAi_primary | |||
| T21G5.5c.1 | Inferred_automatically | RNAi_primary | |||
| T21G5.5b.2 | Inferred_automatically | RNAi_primary | |||
| T21G5.5a.1 | Inferred_automatically | RNAi_primary | |||
| T21G5.5d.1 | Inferred_automatically | RNAi_primary | |||
| Species | Caenorhabditis elegans | ||||
| Interaction | WBInteraction000520189 | ||||
| Reference | WBPaper00041641 | ||||
| Phenotype | WBPhenotype:0001404 | Remark | asd-2 RNAi in conjunction with the asd-2(yb1540) mutation restored sinusoidal locomotion to the unc-60(su158) mutant and greatly improved the actin filament organization in body wall muscles, as determined by tetramethylrhodamine-phalloidin staining. asd-2 RNAi/yb1540 also resulted in ectopic expression of endogenous UNC-60A in body wall muscles in the unc-60(su158) mutant, as determined by UNC-60A antibody staining. | ||
| WBPhenotype:0001570 | Remark | asd-2 RNAi in conjunction with the asd-2(yb1540) mutation restored sinusoidal locomotion to the unc-60(su158) mutant and greatly improved the actin filament organization in body wall muscles, as determined by tetramethylrhodamine-phalloidin staining. asd-2 RNAi/yb1540 also resulted in ectopic expression of endogenous UNC-60A in body wall muscles in the unc-60(su158) mutant, as determined by UNC-60A antibody staining. | |||
| WBPhenotype:0004018 | Remark | asd-2 RNAi in conjunction with the asd-2(yb1540) mutation restored sinusoidal locomotion to the unc-60(su158) mutant and greatly improved the actin filament organization in body wall muscles, as determined by tetramethylrhodamine-phalloidin staining. asd-2 RNAi/yb1540 also resulted in ectopic expression of endogenous UNC-60A in body wall muscles in the unc-60(su158) mutant, as determined by UNC-60A antibody staining. | |||
| Remark | (Figure 5F, S3D) asd-2 RNAi. Exact sequence used for RNAi not stated by authors, Ahringer laboratory clone used for curation. | ||||
| Method | RNAi |
