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WormBase Tree Display for RNAi: WBRNAi00092334

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Name Class

WBRNAi00092334HomolHomol_homolC32D5:RNAi
Sequence_infoPCR_productsjj_C32D5.9
ExperimentLaboratoryXH
Date16 May 2012 00:00:00
Genotypeeri-1(mg366); mboa-7(tm3536)
TreatmentFor the assessment of growth, adult P0 worms were allowed to lay eggs for 2-3 hours on RNAi plates, and after incubation at 20 degrees Celsius for 72 hours, the F1 progeny was transferred to fresh RNAi plates. Then, the F1 adult worms were allowed to lay eggs for 2-3 hours on RNAi plates, and their F2 progeny was observed. Worms that displayed small, skinny and pale appearance and did not grow to adult size after 60 h of growth were counted as growth defects.
Delivered_byBacterial_feeding
InhibitsPredicted_geneC32D5.9Inferred_automaticallyRNAi_primary
GeneWBGene00002980Inferred_automaticallyRNAi_primary
TranscriptC32D5.9.1Inferred_automaticallyRNAi_primary
SpeciesCaenorhabditis elegans
InteractionWBInteraction000520165
ReferenceWBPaper00041372
PhenotypeWBPhenotype:0000030RemarkRNAi resulted in growth defects
PenetranceRange38
Remark(Figure 5, Table S3) lgg-1 RNAi. Authors state RNAi clones obtained from the Ahringer and Vidal laboratories, Ahringer clone used for curation. If Ahringer clone not available, Vidal laboratory clone used for curation.
MethodRNAi