WormBase Tree Display for RNAi: WBRNAi00088876

WBRNAi00088876 Homol: Homol_homol: K11D12:RNAi
  Sequence_info: PCR_product: mv_K11D12.9
  Experiment: Laboratory: ALF
    Date: 21 Mar 2011 00:00:00
    Genotype: (daf-2(e1371); spe-9(hc88); fer-15(b26); rde-1(ne219); bafIs110 (myo-3p:rde-1))
    Treatment: RNAi treatment was performed by feeding animals double-stranded RNA-producing bacteria as described previously except that we used a 4x concentrated RNAi culture to spot plates. After drying, 50-60 eggs isolated by hypochlorite treatment were added to the RNAi plates and kept at 20 degrees Celsius until the worms reached the L4 stage before being transferred to 25 degrees Celsius. This is the nonpermissive temperature for adult fertility and creates a synchronized adult population.
    Temperature: 25
    Delivered_by: Bacterial_feeding
  Inhibits: Predicted_gene: K11D12.9 Inferred_automatically: RNAi_primary
    Gene: WBGene00019649 Inferred_automatically: RNAi_primary
    Transcript: K11D12.9.1 Inferred_automatically: RNAi_primary
  Species: Caenorhabditis elegans
  Reference: WBPaper00038430
  Phenotype: WBPhenotype:0001213 Remark: Muscle-specific RNAi of K11D12.9 in the ALF112 background (daf-2(e1371); spe-9(hc88); fer-15(b26); rde-1(ne219); bafIs110 (myo-3p::rde-1)) causes a more rapid decline of mobility with age compared to RNAi control animals.
      EQ_annotations: Life_stage: WBls:0000041 PATO:0000460
    WBPhenotype:0001739 Remark: Muscle-specific RNAi of K11D12.9 in the ALF112 background (daf-2(e1371); spe-9(hc88); fer-15(b26); rde-1(ne219); bafIs110 (myo-3p::rde-1)) causes a more rapid decline of mobility with age compared to RNAi control animals.
      EQ_annotations: Life_stage: WBls:0000041 PATO:0000460
  Remark: Muscle-specific K11D12.9 RNAi
  Method: RNAi