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WormBase Tree Display for RNAi: WBRNAi00087850

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Name Class

WBRNAi00087850HomolHomol_homolZK678:RNAi
Sequence_infoPCR_productsjj_ZK678.1
ExperimentLaboratoryOL
Date12 Feb 2010 00:00:00
Genotypelin-15B(n744)
TreatmentFor lin-15A(RNAi) quantitative RT-PCR experiments ?1000 synchronised L3 staged lin-15B(n744) worms were placed on RNAi plates seeded with the respective RNAi clone. The P0 mothers were washed off the plates and bleached once they had been on RNAi for ?54 h. The eggs were placed directly back onto RNAi plates seeded with the appropriate RNAi clone and the F1 worms harvested after 48 h.
Delivered_byBacterial_feeding
InhibitsPredicted_geneZK678.1Inferred_automaticallyRNAi_primary
GeneWBGene00023498Inferred_automaticallyRNAi_primary
TranscriptZK678.1.1Inferred_automaticallyRNAi_primary
SpeciesCaenorhabditis elegans
InteractionWBInteraction000517930
ReferenceWBPaper00035979
PhenotypeWBPhenotype:0000136Remarklin-15A(RNAi) in the lin-15B(n744) background results in overexpression of the lin-3 gene, as indicated by RT-PCR.
MethodRNAi