WormBase Tree Display for RNAi: WBRNAi00086321
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| WBRNAi00086321 | Homol | Homol_homol | Y73B6BL:RNAi | ||
|---|---|---|---|---|---|
| Sequence_info | DNA_text | atggcttcggtggcggcgtggctgccgttcgcgagagccgccgctatcggatgggttcccatttcgagacagcccatgccacaggccccagttgcaatacaggccaaagacctcgcggttgatcatgtctctgacgaaaaactagcaatcaacatatcaggacgaaggttcgaaacgtggaagaacacgttggaaaagttcccagagactctattggggtcaaatgaaaaagagttcttttatgatgaagacactggggaatactttttcgatagggatccggacatattccgacacattctcacattttatagaacgggaaaattgcattatccccgtcatgaatgtcttgttgcatacgacgaagagctcagtttctttggaataatgcctgatttgatatcagattgttgctatgaagactataaagataaaaaacgagaaaatcaagaaagattacaagaagaacgtgtagaaaatgcggatatatcaacattaaaattgtctcttaaagagaaaatgtgggcggcattcgagaatccgcacacaacatcaatagcacttgtattttattatgtcattggattttttattgctgtatcagtgatgtgtaatatagtggaaacaataccttgtgggtatgaggataatgtgtctgtgacgtgtggagaagcctatgaagagcaattctttgttatcgatacagcgtgtgttatcatttttacgattgagtactttttacggttgatttctgcgccggaccgaatcaaatttatgaggtcaataatgagtgtaatcgatgtgattgccataatgccttactacgttagtttggtactaactgacaataaggatgtatccggcttgtttgtcactttaagagttttccgtgtcttccgaatattcaaattctcacgacattcccaaggtcttcgaattctgggttacactcttaaatcgtgtgcaagtgaacttggattccttgtattttcactggcaatggcaattatcatatttgccactattatgtattatgcggagaaaaaggtcgacgcgacgagatttacctcgattccgtcagctttctggtatacaattgtgactctaacaactcttggatacggagacatggttccatcaacaataatgggtaaaatagttggaggagtatgctcactgtctggagtgttggttattgctcttccagttccagttattgtcagcaatttctctcgaatttatcatcaaaatcagagagctgacaagagaaaagcacagaagaaagcccgtcttgctagaattcggatcgttaaaaacgcgtctggactagcattattcaataagaagagagctcatgaagctcggatgctagcatttgaacagggtcatctctctttcgatgctctccgagacgaagatatcttcgaaatccaacatcatcatcttcttcagtgtctagaaaaagcgacggaacgtgaatttgttgaatcagaagtgatgtttgaaggtggaagaaatactcctccgcccagtgaaacagcttcattgaaaggaaaaacaaagcgtaaaaggcgattatgctgtgtttcaaaagaaaatgaagaaatggaagagttggatcgagagactcgtgtaacattcaatcagaatatggatcagatttgtgaattaccaaaacctgatgaaatggaactcaatcatcgaaataatgacaaaatttgcgtatcgcaactatggaaatataaaattgaagtaaaattaactttttatgtgcagttttttttttttcaattttccaggtctgtcgataaagttttatttattttctaa | |||
| Experiment | Laboratory | LY | |||
| Date | 09 Aug 2006 00:00:00 | ||||
| Treatment | Double-stranded RNA (dsRNA) was synthesized byusing standard methods. Cultured myocytes were plated in L-15 control medium or L-15 medium containing 15 micrograms/ml dsRNA final volume. One hour after plating, the dsRNA was diluted to a final concentration of 5 micrograms/ml. Media containing the dsRNA were replaced each day. Electrophysiological experiments were performed 24 days after plating the cells. | ||||
| Delivered_by | Soaking | ||||
| Inhibits | Predicted_gene | Y73B6BL.19 | Inferred_automatically | RNAi_primary | |
| Gene | WBGene00022240 | Inferred_automatically | RNAi_primary | ||
| Transcript | Y73B6BL.19.1 | Inferred_automatically | RNAi_primary | ||
| Species | Caenorhabditis elegans | ||||
| Reference | WBPaper00028359 | ||||
| Phenotype_not_observed | WBPhenotype:0001315 | Remark | There was no significant difference in the amplitudes of normalized sustained currents between N2 myocytes and the shl-1 RNAi-treated myocytes | ||
| Remark | Exact sequence used for RNAi not stated by authors, spliced coding region sequence of gene used for curation. | ||||
| Method | RNAi |
