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WormBase Tree Display for RNAi: WBRNAi00086077

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Name Class

WBRNAi00086077HomolHomol_homolF10E9:RNAi
Sequence_infoPCR_productWBPaper00005739:F10E9_1.1
ExperimentLaboratoryTH
Date04 Feb 2003 00:00:00
TreatmentGFP-gamma-tubulin and GFP-histone used as markers.
Delivered_byInjection
InhibitsPredicted_geneF10E9.8Inferred_automaticallyRNAi_primary
GeneWBGene00004726Inferred_automaticallyRNAi_primary
TranscriptF10E9.8.1Inferred_automaticallyRNAi_primary
SpeciesCaenorhabditis elegans
ReferenceWBPaper00005739
PhenotypeWBPhenotype:0001903RemarkCentrosome duplication fails prior to the second mitotic division in sas-4(RNAi) embryos. In all sas-4((RNAi) embryos bipolar spindles with wild-type levels of gamma-tubulin fluorescence at the spindle poles formed during the first mitotic division. However, only a single focus of gamma-tubulin fluorescence was observed in each daughter cell during the second mitotic division. These single centrosomes accumulated gamma -tubulin normally and organized monopolar spindles.
MethodRNAi