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WormBase Tree Display for RNAi: WBRNAi00083298

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Name Class

WBRNAi00083298HomolHomol_homolY71F9AL:RNAi
Sequence_infoDNA_textcagaaccgctttccacgctgaagccgaagatcatgagcagctagaaaatgcgaaccgccgacaagccgaaaaggagagattccgccaaatttggcatctatttctgacgcatttggagcaagatccacggaaaactggaagtttctggctgcaagagctgcctgtgttgctccgcgaggtttcgagtttcgatgaggatattttgggattcggagcgcttgatggtgaggagcaattcgatttttttgcagttgcaggagaagccgaagtagacgggaacaagtatccatcattgtcgggaatgtgtaaattgtcgtttttcattatatttttcatgtttttgctggtattaatttg
ExperimentLaboratoryYM
Date08 Jan 2003 00:00:00
GenotypeAJM-1::GFP
TreatmentL4 larvae were washed with M9 several times and placed on an NGM plate without OP50 for 1 hour. Five worms were selected and immersed in RNA solution for 24 hours and then allowed to recover by incubation at 20C on NGM plates for 36 hours before observation.
Delivered_bySoaking
InhibitsPredicted_geneY71F9AL.6Inferred_automaticallyRNAi_primary
GeneWBGene00022111Inferred_automaticallyRNAi_primary
TranscriptY71F9AL.6.1Inferred_automaticallyRNAi_primary
SpeciesCaenorhabditis elegans
ReferenceWBPaper00005843
PhenotypeWBPhenotype:0000703EQ_annotationsLife_stageWBls:0000003PATO:0000460
WBPhenotype:0001908RemarkHypodermal cells of the developing embryo failed to migrate properly toward the ventral midline, thus resulting in a ventral enclosure defect
EQ_annotationsLife_stageWBls:0000003PATO:0000460
RemarkAuthors report that clone yk170f5 was used to target arx-1 for knockdown, which maps to an intron of the arx-1 gene and an exon of the gene Y71F9AL.6; results should be interpreted with caution
MethodRNAi