WormBase Tree Display for RNAi: WBRNAi00081144
expand all nodes | collapse all nodes | view schema
| WBRNAi00081144 | Homol | Homol_homol | F22B5:RNAi | ||
|---|---|---|---|---|---|
| Sequence_info | PCR_product | sjj_F22B5.10 | |||
| Experiment | Laboratory | DM | |||
| Date | 26 May 2009 00:00:00 | ||||
| Strain | WBStrain00033508 | ||||
| Treatment | Authors screened for defects in myosin localization, using the MYO-3::GFP expressing strain RW1596 grown on various RNAi feeding bacterial strains. Briefly, approximately 30 synchronized L1s (P0) animals were spotted onto each RNAi plate. These plates were incubated until the young adult stage (,6068 hours), then 2 P0 were transferred onto fresh plates. To synchronize the F1 generation, the adult animals were removed after an 18-hour egg-laying period. Embryos were allowed to hatch and develop to the L4/young adult stage (36 hours). 1040 worms from each plate were screened for body wall muscle defects via MYO-3::GFP expression. | ||||
| Temperature | 20 | ||||
| Delivered_by | Bacterial_feeding | ||||
| Inhibits | Predicted_gene | F22B5.10 | Inferred_automatically | RNAi_primary | |
| Gene | WBGene00009045 | Inferred_automatically | RNAi_primary | ||
| Transcript | F22B5.10.1 | Inferred_automatically | RNAi_primary | ||
| Species | Caenorhabditis elegans | ||||
| Reference | WBPaper00034662 | ||||
| Phenotype | WBPhenotype:0001569 | Remark | General myofilament disorganziation with minor disruptions and or aggregations. | ||
| Method | RNAi |
