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WormBase Tree Display for RNAi: WBRNAi00077986

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Name Class

WBRNAi00077986HomolHomol_homolCHROMOSOME_IV:RNAi
Sequence_infoDNA_textgacgagctgctcaaagagaacaatgagctgaaaagctcatcggtctcatcgggaaaagcctcaccatcaccggcagagtcgaggagctctccgaagcttgtcgagacagtagtcgctccagtatcaggagcccggaagcggaagcccaaggagcggagcccaccggcagctgcgagtccacttccagacttttcgaatttgatgaacggattcatgtttgatcctctgaatatgtcaaatccaaatggaatgatgcaattattatcaatggttcaacagcagcagcagcagcaacaacaccaccagcacattgagaatcaacaatccgtgagcccaccacaatcgaaatccgtgaaaattgaagatccaatggatcaggatgtgaaacaggaagagtcggagcgatccgatatcccgacggctacagaggcgcagaaccttttggatgcactaacagcacagtttagcagcaacggacaggcgacctccaccacatctccaccatcatcatcatcccaagtacaagcggtcatcgaagcagtcgccacaccatcatcccaaagccaagacagttcaatgtttgagaaaaccgagacgagtggtgatccgaacgcggcgcgatgctccaactgccgaaccgacaagacaaccgcgtggcgacgtgacgccgaggggaagcttgtctgcaatccgtgtgggctctactatcgattgcacaaggttcgccgaccaatagaaatgcgcaaaaaccacatccaacaacgctaccgccgaaagaacaaggaaaaagagtcgtcggcggcaacacagatcttcaaccaactcctcacacaaatgccgacgatggcgaccggcggagtgagcactgatggagccatcaacacattcaatttactcgagcaaatctcgcaattc
ExperimentLaboratoryJR
Date16 May 2001 00:00:00
Delivered_byInjection
InhibitsPredicted_geneF55A8.1aInferred_automaticallyRNAi_primary
F55A8.1bInferred_automaticallyRNAi_primary
GeneWBGene00001186Inferred_automaticallyRNAi_primary
TranscriptF55A8.1b.1Inferred_automaticallyRNAi_primary
F55A8.1a.2Inferred_automaticallyRNAi_primary
F55A8.1a.1Inferred_automaticallyRNAi_primary
SpeciesCaenorhabditis elegans
ReferenceWBPaper00004833
PhenotypeWBPhenotype:0000811RemarkSeam cells fail to differentiate properly. Authors used various markers to observe the expression of seam specific NR genes. Expression of three NR reporters, nhr-75 ::GFP, nhr-81 ::GFP,and NHR-82::GFP, was undetectable in all elt-5(RNAi) embryos examined. In contrast, expression of two NR reporters, nhr-73 ::GFP and nhr-74 ::GFP, was only slightly affected, both in terms of the fraction of expressing animals and the level of GFP signal.. The remaining three NR reporters, nhr-72 ::GFP, nhr-77 ::GFP, and nhr-89 ::GFP, gave intermediate results; these were expressed much less frequently in elt-5(RNAi) embryos than in wild-type embryos, and expression was barely detectable in only a few cells for those embryos showing any expression.
MethodRNAi