WormBase Tree Display for RNAi: WBRNAi00070137

WBRNAi00070137 Homol: Homol_homol: K09D9:RNAi
  Sequence_info: PCR_product: sjj_K09D9.1
  Experiment: Laboratory: JF
    Date: 25 Jun 2007 00:00:00
    Genotype: mtl-2(gk125)
    Treatment: Synchronized L1 larva were put on standard NGM plates with E. coli OP50 as food for approximately 31 h and allowed to develop into L4 larva. About 25 early L4 nematodes were then transferred to each well of a 6-well NGM plate, containing 1 mM IPTG and 25 mcg/ml carbenicillin, seeded with different RNAi expressing bacteria. Plates were then incubated at 20C for approximately 40 h. After the initial feeding period, 2 gravid adults from each well were then transferred to test plates containing RNAi bacteria and either 0 or 100 mcM cadmium. Each exposure was performed in triplicate. Control RNAi (empty vector) exposures were also performed for each cadmium treatment. Adults were allowed to lay eggs for approximately 12 h before removal. F1 nematodes were then grown on test plates for three additional days at 20C. At the end of the 3 day growth period, F1 nematodes were washed off the 6-well plates using M9 buffer and transferred onto 96-well plates. Body size measurements, as represented by nematode body length (time of flight (TOF)) were measured using the COPAS Biosort.
    Temperature: 20
    Delivered_by: Bacterial_feeding
  Inhibits: Predicted_gene: K09D9.1 Inferred_automatically: RNAi_primary
    Gene: WBGene00019564 Inferred_automatically: RNAi_primary
    Transcript: K09D9.1.1 Inferred_automatically: RNAi_primary
  Species: Caenorhabditis elegans
  Reference: WBPaper00030811
  Phenotype: WBPhenotype:0000031 Remark: Difference between normalized mean body lengths of RNAi-treated animals exposed and not exposed to cadmium = 0.24
    WBPhenotype:0001655 Remark: Medium protective effects against cadmium toxicity.
  Method: RNAi